|
| Status |
Public on Dec 16, 2015 |
| Title |
EP33W whole blood Control genomic DNA |
| Sample type |
genomic |
| |
|
| Source name |
whole blood genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
disease state: Control
tissue: whole blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
All genomic DNA were extracted from either whole blood or fibroblast using standard procedures. 1 microgram of genomic DNA was sodium bisulfite modified using the Qiagen EZ DNA Methylation kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol. Modified genomic DNA was then processed and analyzed on the Infinium HumanMethylation450 BeadChip from Illumina (Illumina 450K) according to the manufacturer’s protocol.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Using an Illumina HiScan
|
| Data processing |
Using an Illumina HiScan
|
| |
|
| Submission date |
Oct 28, 2015 |
| Last update date |
Dec 16, 2015 |
| Contact name |
Rosanna Weksberg |
| Organization name |
The Hospital for Sick Children
|
| Department |
Genetics and Genome Biology
|
| Lab |
Weksberg Lab
|
| Street address |
555 University Ave.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE74432 |
NSD1 Mutations Generate a Genome-Wide DNA Methylation Signature |
|
