|
Status |
Public on Sep 01, 2011 |
Title |
normal mammary tissue rep4 |
Sample type |
RNA |
|
|
Source name |
mammary tissue harvested from wild type FVB mouse
|
Organism |
Mus musculus |
Characteristics |
strain: FVB tissue: primary mammary tissue
|
Growth protocol |
Primary tumors were harvested from mouse, flash frozen and prepared for RNA. M6 and M6C cells were grown in DMEM high glucose with 5% FBS, Na Pyruvate, and Pen/Strep prior to harvest
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of RNA was performed according to manufacturers protocol
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared accorded to standard affymetrix protocol
|
|
|
Hybridization protocol |
cRNA were hybridized onto GeneChip Mouse Genome 430A 2.0 Array.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000.
|
Description |
gene expression data
|
Data processing |
Data were collected using Affymetrix GCOS software and then processed with RMA and quantile normalization using Bioconductor affy package (http://www.bioconductor.org/packages/release/bioc/html/affy.html).
|
|
|
Submission date |
Nov 18, 2010 |
Last update date |
Sep 01, 2011 |
Contact name |
Jeffrey E Green |
E-mail(s) |
JEGreen@mail.nih.gov
|
Phone |
3014355193
|
Fax |
3014968709
|
Organization name |
NCI
|
Street address |
Rm 4054, 37 Convent Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE25488 |
Expression data from C3(1)Tag mammary tumors, tumor derived cell line (M6) and normal mammary tissue (FVB) |
|