|
| Status |
Public on Sep 01, 2011 |
| Title |
normal mammary tissue rep5 |
| Sample type |
RNA |
| |
|
| Source name |
mammary tissue harvested from wild type FVB mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
tissue: primary mammary tissue
|
| Growth protocol |
Primary tumors were harvested from mouse, flash frozen and prepared for RNA. M6 and M6C cells were grown in DMEM high glucose with 5% FBS, Na Pyruvate, and Pen/Strep prior to harvest
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of RNA was performed according to manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared accorded to standard affymetrix protocol
|
| |
|
| Hybridization protocol |
cRNA were hybridized onto GeneChip Mouse Genome 430A 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000.
|
| Description |
gene expression data
|
| Data processing |
Data were collected using Affymetrix GCOS software and then processed with RMA and quantile normalization using Bioconductor affy package (http://www.bioconductor.org/packages/release/bioc/html/affy.html).
|
| |
|
| Submission date |
Nov 18, 2010 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Jeffrey E Green |
| E-mail(s) |
JEGreen@mail.nih.gov
|
| Phone |
3014355193
|
| Fax |
3014968709
|
| Organization name |
NCI
|
| Street address |
Rm 4054, 37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE25488 |
Expression data from C3(1)Tag mammary tumors, tumor derived cell line (M6) and normal mammary tissue (FVB) |
|
