Skip to main content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
J Virol. 1986 May; 58(2): 700–703.
doi: 10.1128/jvi.58.2.700-703.1986
PMCID: PMC252967
PMID: 2422398

Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7.

P A Offit, R D Shaw, and H B Greenberg
Copyright and License information PMC Disclaimer

Abstract

Monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against virulent rotavirus challenge. Monoclonal antibodies to two distinct epitopes of vp7 of simian rotavirus strain RRV neutralized RRV in vitro and passively protected suckling mice against RRV challenge. A monoclonal antibody directed against vp3 of porcine rotavirus strain OSU neutralized three distinct serotypes in vitro (OSU, RRV, and UK) and passively protected suckling mice against OSU, RRV, and UK virus-induced diarrhea. The role of vp3 in eliciting protection against heterotypic rotavirus challenge should be considered when developing a vaccine with cloned rotavirus genes. Alternatively, immunization with a reassortant rotavirus containing vp3 and vp7 from two antigenically distinct rotavirus parents might protect against diarrhea induced by two or more rotavirus serotypes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (782K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

 
icon of scanned page 700
700
icon of scanned page 701
701
icon of scanned page 702
702
icon of scanned page 703
703
 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  • Arias CF, López S, Bell JR, Strauss JH. Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence. J Virol. 1984 May;50(2):657–661. [PMC free article] [PubMed] [Google Scholar]
  • Balachandran N, Bacchetti S, Rawls WE. Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2. Infect Immun. 1982 Sep;37(3):1132–1137. [PMC free article] [PubMed] [Google Scholar]
  • Black RE, Merson MH, Rahman AS, Yunus M, Alim AR, Huq I, Yolken RH, Curlin GT. A two-year study of bacterial, viral, and parasitic agents associated with diarrhea in rural Bangladesh. J Infect Dis. 1980 Nov;142(5):660–664. [PMC free article] [PubMed] [Google Scholar]
  • Boere WA, Benaissa-Trouw BJ, Harmsen M, Kraaijeveld CA, Snippe H. Neutralizing and non-neutralizing monoclonal antibodies to the E2 glycoprotein of Semliki Forest virus can protect mice from lethal encephalitis. J Gen Virol. 1983 Jun;64(Pt 6):1405–1408. [PubMed] [Google Scholar]
  • Both GW, Mattick JS, Bellamy AR. Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence. Proc Natl Acad Sci U S A. 1983 May;80(10):3091–3095. [PMC free article] [PubMed] [Google Scholar]
  • Davidson GP, Bishop RF, Townley RR, Holmes IH. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. [PubMed] [Google Scholar]
  • Dyall-Smith ML, Holmes IH. Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res. 1984 May 11;12(9):3973–3982. [PMC free article] [PubMed] [Google Scholar]
  • Glass RI, Keith J, Nakagomi O, Nakagomi T, Askaa J, Kapikian AZ, Chanock RM, Flores J. Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology. 1985 Mar;141(2):292–298. [PubMed] [Google Scholar]
  • Greenberg H, McAuliffe V, Valdesuso J, Wyatt R, Flores J, Kalica A, Hoshino Y, Singh N. Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983 Jan;39(1):91–99. [PMC free article] [PubMed] [Google Scholar]
  • Greenberg HB, Valdesuso J, van Wyke K, Midthun K, Walsh M, McAuliffe V, Wyatt RG, Kalica AR, Flores J, Hoshino Y. Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. J Virol. 1983 Aug;47(2):267–275. [PMC free article] [PubMed] [Google Scholar]
  • Greenberg HB, Vo PT, Jones R. Cultivation and characterization of three strains of murine rotavirus. J Virol. 1986 Feb;57(2):585–590. [PMC free article] [PubMed] [Google Scholar]
  • GREENWOOD FC, HUNTER WM, GLOVER JS. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. [PMC free article] [PubMed] [Google Scholar]
  • Hoshino Y, Wyatt RG, Greenberg HB, Flores J, Kapikian AZ. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984 May;149(5):694–702. [PubMed] [Google Scholar]
  • Kalica AR, Greenberg HB, Wyatt RG, Flores J, Sereno MM, Kapikian AZ, Chanock RM. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. [PubMed] [Google Scholar]
  • Kapikian AZ, Kim HW, Wyatt RG, Cline WL, Arrobio JO, Brandt CD, Rodriguez WJ, Sack DA, Chanock RM, Parrott RH. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children. N Engl J Med. 1976 Apr 29;294(18):965–972. [PubMed] [Google Scholar]
  • Matsuno S, Inouye S, Kono R. Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977 Jan;5(1):1–4. [PMC free article] [PubMed] [Google Scholar]
  • Offit PA, Blavat G. Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986 Jan;57(1):376–378. [PMC free article] [PubMed] [Google Scholar]
  • Offit PA, Clark HF. Protection against rotavirus-induced gastroenteritis in a murine model by passively acquired gastrointestinal but not circulating antibodies. J Virol. 1985 Apr;54(1):58–64. [PMC free article] [PubMed] [Google Scholar]
  • Offit PA, Clark HF. Maternal antibody-mediated protection against gastroenteritis due to rotavirus in newborn mice is dependent on both serotype and titer of antibody. J Infect Dis. 1985 Dec;152(6):1152–1158. [PubMed] [Google Scholar]
  • Offit PA, Clark HF, Kornstein MJ, Plotkin SA. A murine model for oral infection with a primate rotavirus (simian SA11). J Virol. 1984 Jul;51(1):233–236. [PMC free article] [PubMed] [Google Scholar]
  • Offit PA, Clark HF, Plotkin SA. Response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization. Infect Immun. 1983 Oct;42(1):293–300. [PMC free article] [PubMed] [Google Scholar]
  • Offit PA, Clark HF, Stroop WG, Twist EM, Plotkin SA. The cultivation of human rotavirus, strain 'Wa', to high titer in cell culture and characterization of the viral structural polypeptides. J Virol Methods. 1983 Jul;7(1):29–40. [PubMed] [Google Scholar]
  • Orvell C, Grandien M. The effects of monoclonal antibodies on biologic activities of structural proteins of Sendai virus. J Immunol. 1982 Dec;129(6):2779–2787. [PubMed] [Google Scholar]
  • Rector JT, Lausch RN, Oakes JE. Use of monoclonal antibodies for analysis of antibody-dependent immunity to ocular herpes simplex virus type 1 infection. Infect Immun. 1982 Oct;38(1):168–174. [PMC free article] [PubMed] [Google Scholar]
  • Sheridan JF, Smith CC, Manak MM, Aurelian L. Prevention of rotavirus-induced diarrhea in neonatal mice born to dams immunized with empty capsids of simian rotavirus SA-11. J Infect Dis. 1984 Mar;149(3):434–438. [PubMed] [Google Scholar]
  • Stuker G, Oshiro LS, Schmidt NJ. Antigenic comparisons of two new rotaviruses from rhesus monkeys. J Clin Microbiol. 1980 Feb;11(2):202–203. [PMC free article] [PubMed] [Google Scholar]
  • Taniguchi K, Urasawa S, Urasawa T. Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. J Gen Virol. 1985 May;66(Pt 5):1045–1053. [PubMed] [Google Scholar]
  • Vesikari T, Isolauri E, Delem A, d'Hondt E, André FE, Beards GM, Flewett TH. Clinical efficacy of the RIT 4237 live attenuated bovine rotavirus vaccine in infants vaccinated before a rotavirus epidemic. J Pediatr. 1985 Aug;107(2):189–194. [PubMed] [Google Scholar]
  • Vesikari T, Isolauri E, D'Hondt E, Delem A, André FE, Zissis G. Protection of infants against rotavirus diarrhoea by RIT 4237 attenuated bovine rotavirus strain vaccine. Lancet. 1984 May 5;1(8384):977–981. [PubMed] [Google Scholar]
  • Walsh EE, Schlesinger JJ, Brandriss MW. Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infect Immun. 1984 Feb;43(2):756–758. [PMC free article] [PubMed] [Google Scholar]
  • Walsh JA, Warren KS. Selective primary health care: an interim strategy for disease control in developing countries. N Engl J Med. 1979 Nov 1;301(18):967–974. [PubMed] [Google Scholar]
  • Webster RG, Laver WG. Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology. 1980 Jul 15;104(1):139–148. [PubMed] [Google Scholar]
  • Wiktor TJ, Koprowski H. Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3938–3942. [PMC free article] [PubMed] [Google Scholar]
  • Wyatt RG, Kapikian AZ, Mebus CA. Induction of cross-reactive serum neutralizing antibody to human rotavirus in calves after in utero administration of bovine rotavirus. J Clin Microbiol. 1983 Sep;18(3):505–508. [PMC free article] [PubMed] [Google Scholar]
  • Wyatt RG, Mebus CA, Yolken RH, Kalica AR, James HD, Jr, Kapikian AZ, Chanock RM. Rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced by bovine virus. Science. 1979 Feb 9;203(4380):548–550. [PubMed] [Google Scholar]
  • Zissis G, Lambert JP, Marbehant P, Marissens D, Lobmann M, Charlier P, Delem A, Zygraich N. Protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge. J Infect Dis. 1983 Dec;148(6):1061–1068. [PubMed] [Google Scholar]
Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)
-