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Published online 2007 Oct 29. doi: 10.1359/JBMR.071030
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FIG. 4

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Effects of receptor endocytosis on BMP signaling. Cells were preincubated for 1 h in potassium-free medium (No-K) to block internalization through clathrin-coated pits (top) or in medium containing 4 mM β-cyclodextrin (β-CycDex) to block caveolin-mediated internalization (bottom) and treated with BMP4 (100 ng/ml) for 1.5 h. ID1 mRNA was quantified by real-time PCR. Statistical analysis (ANOVA): ID1 expression, *BMP4 vs. untreated cells and BMP4 vs. No K + BMP4 (p < 0.01).

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