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Published online 2009 Dec 14. doi: 10.1073/pnas.0905767106
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Fig. 5.

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Functional dependence of Dnmt3L on binding to unmethylated histone tails. (A) Western detection of Flag-tagged Dnmt3L PHD-mutants coexpressed with HA-Dnmt3a in yeast. (B) Peptide binding assay for the Dnmt3L PHD mutant proteins purified from yeast. Note that only the wild-type Dnmt3L control could bind to the H3 peptide that lacked modification at lysine 4. WDR5 (WD repeat domain 5) known to bind H3 terminus with unmethylated arginine 2 (40) was used as a control. (C) Requirement of Dnmt3L's PHD function to promote the Dnmt3a methylation activity.

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