Figure 4

Kinetics of proteinase-activated receptor 2 (PAR₂) agonist peptide (AP)-mediated differential modulation of Toll-like receptor 3 (TLR3) responses in human lung epithelial cell line. A549 cells were treated with medium (closed circles), PAR₂ AP (100 µM; open circles), poly I:C (pI:C; 100 µg ml⁻¹; closed squares), or both (open squares/dotted lines) over the indicated time. mRNA was subjected to quantitative real-time PCR (qPCR) analysis. Relative gene expression is presented as the mean ± s.d., representative of three separate experiments. * p<0.05.