Figure 6

Mib1 could rescue the neuronal maturation deficits associated with miR-137 overexpression in vitro. (A) Overexpression of Mib1 enhanced dendritic length, whereas acute knockdown of Mib1 (Mib1 shRNA) reduced dendritic length. Mib1 expression partially rescued the miR-137-mediated reduction in dendritic length in cultured primary neurons. (B) Overexpression of Mib1 enhanced the dendritic complexity of cultured neurons, whereas acute knockdown of Mib1 reduced dendritic complexity. (C) Mib1 could rescue the miR-137-mediated reduction in dendritic complexity (F(1,35) = 18.51, p < 0.001). (D) A hypothetic model illustrating that miR-137 may regulate dendritic morphogenesis in developing neurons, at least in part, by translational regulation of Mib1. (*, p < 0.05; **, p < 0.01; ***, p < 0.001).