Figure 6

A: Representative Western blot of AMPK α₂ in AMPK WT and KD muscles. The absence of a detectable band at the expected molecular weight of ∼63 kDa in KD mice muscles indicates a strongly reduced endogenous α₂ protein expression. Instead the slightly heavier band in KD muscles is detection of the myc-tag transgenic α₂ protein. B: α₁ protein expression in WT and AMPK KD muscles. *: Indicates significant difference between genotype, P<0.001, n = 10. Values are means ± S.E.M. C: ACC Ser²²¹ phosporyaltion in AMPK WT and KD muscle lysates (TA) from chronic saline (white bars) and metformin (black bars) treated mice. *: Indicates significant difference between genotype, P<0.001, n = 11–14. Values are means ± S.E.M.