Figure 3

Effect of TH, TAM, and TH + TAM on the mitochondrial membrane potential of MCF-7 cells. Cells treated with TH alone or in combination with TH (5 μM) for 24 h were stained with mitochondrial-selective JC-1 dye and analyzed by flow cytometry. (a) The dot plots represent the population of mitochondrial membrane-depolarized cells on the lower right (LR) and mitochondrial membrane-polarized cells on the upper right (UR). (b) Bar graphs represent the percentages of polarized and depolarized cells. Data are expressed as mean ± standard deviation from three independent experiments. ^# P < 0.05 significantly different from untreated control. ^σ P < 0.05 significantly different from TAM alone.