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Published online 2004 Mar 15. doi: 10.1073/pnas.0307720101
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Fig. 1.

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Schematic of falcipain-2 gene disruption. The WT falcipain-2 locus, transfection plasmid (pDC-PFP2/GFPA), and integration locus after a single crossover are shown. Thick lines represent regulatory regions, thin dotted lines indicate the plasmid backbone, and thick dotted lines represent the chromosomal DNA. Bent arrows indicate orientations, horizontal arrows represent the locations of primers, and vertical arrows indicate the locations of restriction endonuclease sites. The sizes of restriction fragments are shown in kilobases in parentheses.

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