Fig. 7.

Effects of jasmonic acid (JA) and nitric oxide (NO) on AMI1 expression. (A) Expression level by quantitative (q)RT–PCR of AMI1 at days 3 and 7 in the induction medium of somatic embryogenesis. WT and pgb2 tissue was cultured in medium with altered levels of NO and JA using the pharmacological treatments described in Fig. 2. Values are means ±SE of at least three biological replicates and are normalized to the value of the WT (C) of day 3 set at 1. An asterisk indicates statistically significant differences (P<0.005) from the WT (C) value of the respective day in culture. (B) Expression level by qRT–PCR of AMI1 in WT and aos lines at days 3 and 7 on induction medium. Values are means ±SE of at least three biological replicates and are normalized to the value of the WT of day 3 set at 1. An asterisk indicates statistically significant differences (P<0.005) from the WT value of the respective day in culture. (C) Expression level by qRT–PCR of AMI1 at days 3 and 7 in the induction medium of somatic embryogenesis in the WT, myc2-1, jaz1-1, and 35S:JAZ1 lines. Values are means ±SE of at least three biological replicates and are normalized to the value of the WT of day 3 set at 1. An asterisk indicates statistically significant differences (P<0.005) from the WT value of the respective day in culture.