Fig. 6

a Percentage of CD11b/c⁺ GFP⁺ bone marrow-derived dendritic cells from Sprague-Dawley rats and CD11c⁺ CD11b⁺ GFP⁺ bone marrow-derived dendritic cells from C57BL/6 mice were analyzed via flow cytometry 3 days after transduction with Ad5-CMV-GFP, Ad5-β2m-GFP, or LV-β2m-GFP at MOI 100. b Percentage of CD11b/c⁺ GFP⁺ or CD11c⁺ CD11b⁺ GFP⁺ cells from bone marrow-derived dendritic cells of Sprague-Dawley rats and C57BL/6 mice, respectively, after 3 days post-transduction with Ad5-CMV-GFP, Ad5-β2m-GFP or LV-β2m-GFP at MOI 1, 10, 50 and 100. c Sprague-Dawley rats (n = 8/group) or C57BL/6 (n = 6/group) were immunized i.m. with 1 × 10⁷ IGU of Ad5-CMV-HIV vectors or 5 × 10⁷ TU of LV-β2m-HIV vectors, and the splenocytes were subjected to IFNγ ELISpot assay using pools of 15-mers, overlapping by 10 a.a., after 14 dpi. Horizontal bars represent means. Each dot represents a biological replicate. Statistical significance was determined via Two-way ANOVA with Tukey’s multiple comparison test (***p < 0.001, **p < 0.01).