Production of a Class IIb Bacteriocin with Broad-spectrum Antimicrobial Activity in Lactiplantibacillus plantarum RUB1

doi:10.1007/s12602-021-09815-2

. 2021 Dec;13(6):1820-1832.

doi: 10.1007/s12602-021-09815-2. Epub 2021 Aug 23.

Production of a Class IIb Bacteriocin with Broad-spectrum Antimicrobial Activity in Lactiplantibacillus plantarum RUB1

Aijuan Wu^{1

2}, Yaqian Fu^{1

2}, Lingyu Kong^{1

2}, Qiyuan Shen^{1

2}, Mingxue Liu^{1

2}, Xiaoqun Zeng^{3

4}, Zhen Wu^{1

2}, Yuxing Guo^{1

5}, Daodong Pan^{1

2}

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo, 315211, China.
² Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo, 315211, China. zengxiaoqun@nbu.edu.cn.
⁴ Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, China. zengxiaoqun@nbu.edu.cn.
⁵ Food Science and Nutrition Department, Nanjing Normal University, Nanjing, 210097, China.

PMID: 34423377
DOI: 10.1007/s12602-021-09815-2

Production of a Class IIb Bacteriocin with Broad-spectrum Antimicrobial Activity in Lactiplantibacillus plantarum RUB1

Aijuan Wu et al. Probiotics Antimicrob Proteins. 2021 Dec.

. 2021 Dec;13(6):1820-1832.

doi: 10.1007/s12602-021-09815-2. Epub 2021 Aug 23.

Authors

Aijuan Wu^{1

2}, Yaqian Fu^{1

2}, Lingyu Kong^{1

2}, Qiyuan Shen^{1

2}, Mingxue Liu^{1

2}, Xiaoqun Zeng^{3

4}, Zhen Wu^{1

2}, Yuxing Guo^{1

5}, Daodong Pan^{1

2}

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo, 315211, China.
² Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo, 315211, China. zengxiaoqun@nbu.edu.cn.
⁴ Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315800, China. zengxiaoqun@nbu.edu.cn.
⁵ Food Science and Nutrition Department, Nanjing Normal University, Nanjing, 210097, China.

PMID: 34423377
DOI: 10.1007/s12602-021-09815-2

Abstract

Bacteriocins produced by lactic acid bacteria have potential use as natural food preservatives, which may alleviate current problems associated with the overuse of antibiotics and emerging multi-drug-resistant microbes. In this work, Lactiplantibacillus plantarum RUB1 was found to produce a class IIb bacteriocin with strong antibacterial activity. Except for plnXY encoding putative proteins, L. plantarum RUB1 contains most genes in five operons (plnABCD, plnGHSTUVW, plnMNOP, plnIEF, and plnRLJK) related to bacteriocin synthesis. Adding low (100 and 500 ng/mL) and medium (1 μg/mL) concentrations of PlnA to broth promoted bacteriocin production and upregulated bacteriocin gene plnA, while high concentrations (50 and 200 μg/mL) inhibited expression of these genes. Co-culturing L. plantarum RUB1 with Enterococcus hirae 1003, Enterococcus hirae LWS, Limosilactobacillus fermentum RC4, L. plantarum B6, and even Listeria monocytogenes ATCC 19111 and Staphylococcus aureus ATCC 6538 enhanced bacteriocin activity and expression of bacteriocin-related genes. This study verifies that PlnA can indeed upregulate the expression of bacteriocin genes, and also bacteriocin production can be induced by co-culture with some specific bacteria or their cell-free supernatants. Bacteriocin production by L. plantarum RUB1 is mediated by a quorum sensing mechanism, directly influenced by autoinducing peptide or specific strains. The findings provide new methods and insight into bacteriocin production mechanisms.

Keywords: Bacteriocin production; Broad-spectrum antimicrobials; Co-culturing; Plantaricin A; Quorum sensing.

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References

1. Zendo T (2013) Screening and characterization of novel bacteriocins from lactic acid bacteria. Biosci Biotechnol Biochem 77:893–899. https://doi.org/10.1271/bbb.130014 - DOI - PubMed
1. Maeda M, Shibata A, Biswas G, Korenaga H, Kono T, Itami T, Sakai M (2014) Isolation of lactic acid bacteria from kuruma shrimp (Marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic. Mar Biotechnol (NY) 16:181–192. https://xs.scihub.ltd/ https://doi.org/10.1007/s10126-013-9532-1
1. Juturu V, Wu JC (2018) Microbial production of bacteriocins: latest research development and applications. Biotechnology Adv 8:2187–2200. https://doi.org/10.1016/j.biotechadv.2018.10.007 - DOI
1. Zimina M, Babich O, Prosekov A, Sukhikh S, Noskova S (2020) Overview of global trends in classification, methods of preparation and application of bacteriocins. Antibiotics 9:553–573. https://doi.org/10.3390/antibiotics9090553
1. Turovskiy Y, Kashtanov D, Paskhover B, Chikindas ML (2007) Quorum sensing: fact, fiction, and everything in between. Adv Appl Microbiol 62:191–234. https://doi.org/10.1016/S0065-2164(07)62007-3 - DOI - PubMed - PMC

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[1] Zendo T (2013) Screening and characterization of novel bacteriocins from lactic acid bacteria. Biosci Biotechnol Biochem 77:893–899. https://doi.org/10.1271/bbb.130014 - DOI - PubMed

[2] Zendo T (2013) Screening and characterization of novel bacteriocins from lactic acid bacteria. Biosci Biotechnol Biochem 77:893–899. https://doi.org/10.1271/bbb.130014 - DOI - PubMed

[3] Maeda M, Shibata A, Biswas G, Korenaga H, Kono T, Itami T, Sakai M (2014) Isolation of lactic acid bacteria from kuruma shrimp (Marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic. Mar Biotechnol (NY) 16:181–192. https://xs.scihub.ltd/ https://doi.org/10.1007/s10126-013-9532-1

[4] Maeda M, Shibata A, Biswas G, Korenaga H, Kono T, Itami T, Sakai M (2014) Isolation of lactic acid bacteria from kuruma shrimp (Marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic. Mar Biotechnol (NY) 16:181–192. https://xs.scihub.ltd/ https://doi.org/10.1007/s10126-013-9532-1

[5] Juturu V, Wu JC (2018) Microbial production of bacteriocins: latest research development and applications. Biotechnology Adv 8:2187–2200. https://doi.org/10.1016/j.biotechadv.2018.10.007 - DOI

[6] Juturu V, Wu JC (2018) Microbial production of bacteriocins: latest research development and applications. Biotechnology Adv 8:2187–2200. https://doi.org/10.1016/j.biotechadv.2018.10.007 - DOI

[7] Zimina M, Babich O, Prosekov A, Sukhikh S, Noskova S (2020) Overview of global trends in classification, methods of preparation and application of bacteriocins. Antibiotics 9:553–573. https://doi.org/10.3390/antibiotics9090553

[8] Zimina M, Babich O, Prosekov A, Sukhikh S, Noskova S (2020) Overview of global trends in classification, methods of preparation and application of bacteriocins. Antibiotics 9:553–573. https://doi.org/10.3390/antibiotics9090553

[9] Turovskiy Y, Kashtanov D, Paskhover B, Chikindas ML (2007) Quorum sensing: fact, fiction, and everything in between. Adv Appl Microbiol 62:191–234. https://doi.org/10.1016/S0065-2164(07)62007-3 - DOI - PubMed - PMC

[10] Turovskiy Y, Kashtanov D, Paskhover B, Chikindas ML (2007) Quorum sensing: fact, fiction, and everything in between. Adv Appl Microbiol 62:191–234. https://doi.org/10.1016/S0065-2164(07)62007-3 - DOI - PubMed - PMC

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Production of a Class IIb Bacteriocin with Broad-spectrum Antimicrobial Activity in Lactiplantibacillus plantarum RUB1

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Production of a Class IIb Bacteriocin with Broad-spectrum Antimicrobial Activity in Lactiplantibacillus plantarum RUB1

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