Obesogenic versus ketogenic diets in the regulation of the renin-angiotensin system in rat white and brown adipose tissues
- PMID: 36356378
- DOI: 10.1016/j.nut.2022.111862
Obesogenic versus ketogenic diets in the regulation of the renin-angiotensin system in rat white and brown adipose tissues
Abstract
Objective: The ketogenic diet (KD) has been reported to reverse metabolic dysfunction in obesity. However, it remains unknown how the KD affects the balance between the classical and counterregulatory renin-angiotensin system (RAS) arms in adipose tissue, which carries important implications for metabolic function in adipocytes. The aim of this study was to compare the effects of the obesogenic diet and the KD on RAS balance in white and brown fat.
Methods: Nine male Wistar rats were fed a standard chow (SC), 11 fed a high-fat sucrose-enriched (HFS) obesogenic diet, and 12 a KD. At the end of the 8-wk feeding period, subcutaneous inguinal (Sc Ing), epididymal (Epid), and interscapular brown adipose tissue (iBAT) fat depots were extracted and subsequently used for the measurement of RAS proteins and MasR gene expression.
Results: In SC-fed rats, the Sc Ing fat displayed the highest levels of angiotensin-converting enzyme (ACE)1, but very low levels of angiotensin II types 1 and 2 receptors (AT1R and AT2R) and ACE2. Conversely, the highest levels of ACE2, AT1R, and AT2R were found in iBAT. The HFS diet increased AT1R protein in Sc Ing fat and iBAT, whereas the KD maintained low AT1R levels in these fat depots. However, in Sc Ing and Epid fat depots, the KD elevated AT2R levels and significantly reduced Epid ACE1 levels.
Conclusion: Despite fat depot-specific differences in RAS components, the obesogenic diet promoted the classical RAS arm, whereas the KD attenuated it and enhanced the counterregulatory arm.
Keywords: ACE2; AT(1)R; ERK; MasR; Obesity; Subcutaneous fat; Visceral fat.
Copyright © 2022 Elsevier Inc. All rights reserved.
Similar articles
-
Adipose tissue plasticity mediated by the counterregulatory axis of the renin-angiotensin system: Role of Mas and MrgD receptors.J Cell Physiol. 2024 Jun;239(6):e31265. doi: 10.1002/jcp.31265. Epub 2024 Apr 5. J Cell Physiol. 2024. PMID: 38577921 Review.
-
The ketogenic diet promotes triacylglycerol recycling in white adipose tissue and uncoupled fat oxidation in brown adipose tissue, but does not reduce adiposity in rats.J Nutr Biochem. 2023 Oct;120:109412. doi: 10.1016/j.jnutbio.2023.109412. Epub 2023 Jul 6. J Nutr Biochem. 2023. PMID: 37422170
-
Obesogenic and Ketogenic Diets Distinctly Regulate the SARS-CoV-2 Entry Proteins ACE2 and TMPRSS2 and the Renin-Angiotensin System in Rat Lung and Heart Tissues.Nutrients. 2021 Sep 25;13(10):3357. doi: 10.3390/nu13103357. Nutrients. 2021. PMID: 34684358 Free PMC article.
-
Regulation and Functions of the Renin-Angiotensin System in White and Brown Adipose Tissue.Compr Physiol. 2017 Sep 12;7(4):1137-1150. doi: 10.1002/cphy.c160031. Compr Physiol. 2017. PMID: 28915321 Free PMC article. Review.
-
Thermogenic capacity is antagonistically regulated in classical brown and white subcutaneous fat depots by high fat diet and endurance training in rats: impact on whole-body energy expenditure.J Biol Chem. 2014 Dec 5;289(49):34129-40. doi: 10.1074/jbc.M114.591008. Epub 2014 Oct 25. J Biol Chem. 2014. PMID: 25344623 Free PMC article.
Cited by
-
Effects of very low-calorie ketogenic diet on hypothalamic-pituitary-adrenal axis and renin-angiotensin-aldosterone system.J Endocrinol Invest. 2023 Aug;46(8):1509-1520. doi: 10.1007/s40618-023-02068-6. Epub 2023 Apr 5. J Endocrinol Invest. 2023. PMID: 37017918 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous