Overexpression of vascular endothelin-1 and endothelin-A receptors in a fructose-induced hypertensive rat model
- PMID: 9869011
- DOI: 10.1097/00004872-199816120-00010
Overexpression of vascular endothelin-1 and endothelin-A receptors in a fructose-induced hypertensive rat model
Abstract
Objective: To examine the temporal relationship between hyperinsulinemia and hypertension in the fructose-hypertensive rat model and to study the function of endothelin-1 (ET-1) in fructose-induced hypertension.
Design: Since ET-1 induces insulin resistance in conscious rats, we tested the hypothesis that both hyperinsulinemia and hypertension developed in the fructose-hypertensive rat model might be the sequelae of an elevated tissue content of ET-1 and ET(A) receptors.
Materials and methods: Systolic hypertension was induced within 3 weeks in male Sprague-Dawley rats fed on a fructose-rich diet. After continual monitoring of blood pressure and plasma insulin concentrations, the animals were killed at the end of experiment to determine plasma levels of ET-1, the contractile response of aortic rings to ET-1, and ET-1 and ET(A) receptor gene expressions. In a separate experiment, BQ-610 was administered to lower the effect of ET-1 in rats with fructose-induced hypertension.
Results: Compared with control rats given normal chow, the fructose-fed rats developed systolic hypertension after 3 weeks of the diet (127+/-3.7 versus 110+/-5.5 mmHg, P < 0.01) and hyperinsulinemia both before (1 07.1+/-32.5 versus 48.5+/-14.3 pmol/l, P < 0.005) and after (96.6+/-63.7 versus 50.4+/-5.6 pmol/l, P< 0.05) they became hypertensive. Although plasma ET-1 levels did not differ between the rat groups, aortic ring contraction-concentration curves, indicating vessel contractility in response to ET-1, were significantly greater in these rats than in controls (F1,72 = 12.34, P< 0.00077). Messenger RNA extracted from the tail arteries and blotted with both ET-1 and ET(A) probes showed that fructose-fed rats had greater ET-1 and ET(A)-receptor gene expression than control rats. Concomitant administration of BQ-610 to rats fed on a fructose diet significantly reduced the hypertension. Conclusions These findings suggest that elevated vascular expression of ET-1 and ET(A) receptor genes may mediate the development of hypertension and hyperinsulinemia in rats fed a fructose-rich diet
Similar articles
-
The fructose-fed rat: a review on the mechanisms of fructose-induced insulin resistance and hypertension.Mol Cell Biochem. 2009 Dec;332(1-2):145-59. doi: 10.1007/s11010-009-0184-4. Epub 2009 Jun 18. Mol Cell Biochem. 2009. PMID: 19536638 Review.
-
Endothelin-1 blockade prevents COX2 induction and TXA2 production in the fructose hypertensive rat.Can J Physiol Pharmacol. 2007 Mar-Apr;85(3-4):422-9. doi: 10.1139/y06-088. Can J Physiol Pharmacol. 2007. PMID: 17612651
-
Insulin infusion induces endothelin-1-dependent hypertension in rats.Am J Physiol Endocrinol Metab. 2004 Nov;287(5):E948-54. doi: 10.1152/ajpendo.00536.2003. Epub 2004 Jun 29. Am J Physiol Endocrinol Metab. 2004. PMID: 15226099
-
Gene therapy with human tissue kallikrein reduces hypertension and hyperinsulinemia in fructose-induced hypertensive rats.Hypertension. 2003 Nov;42(5):1026-33. doi: 10.1161/01.HYP.0000097603.55404.35. Epub 2003 Oct 20. Hypertension. 2003. PMID: 14568997
-
Endothelin and endothelin antagonists in hypertension.J Hypertens. 1998 Dec;16(12 Pt 2):1891-5. doi: 10.1097/00004872-199816121-00007. J Hypertens. 1998. PMID: 9886874 Review.
Cited by
-
Reduced basal macrovascular and microvascular cerebral blood flow in young adults with metabolic syndrome: potential mechanisms.J Appl Physiol (1985). 2023 Jul 1;135(1):94-108. doi: 10.1152/japplphysiol.00688.2022. Epub 2023 May 18. J Appl Physiol (1985). 2023. PMID: 37199780 Free PMC article.
-
Fructose Intake, Hypertension and Cardiometabolic Risk Factors in Children and Adolescents: From Pathophysiology to Clinical Aspects. A Narrative Review.Front Med (Lausanne). 2022 Apr 12;9:792949. doi: 10.3389/fmed.2022.792949. eCollection 2022. Front Med (Lausanne). 2022. PMID: 35492316 Free PMC article. Review.
-
The Sweet and Salty Dietary Face of Hypertension and Cardiovascular Disease in Lebanon.Front Physiol. 2022 Jan 25;12:802132. doi: 10.3389/fphys.2021.802132. eCollection 2021. Front Physiol. 2022. PMID: 35153813 Free PMC article. Review.
-
Mesenteric arterial dysfunction in the UC Davis Type 2 Diabetes Mellitus rat model is dependent on pre-diabetic versus diabetic status and is sexually dimorphic.Eur J Pharmacol. 2020 Jul 15;879:173089. doi: 10.1016/j.ejphar.2020.173089. Epub 2020 Apr 19. Eur J Pharmacol. 2020. PMID: 32320701 Free PMC article.
-
Differential effects of high consumption of fructose or glucose on mesenteric arterial function in female rats.J Nutr Biochem. 2018 Jul;57:136-144. doi: 10.1016/j.jnutbio.2018.03.021. Epub 2018 Apr 3. J Nutr Biochem. 2018. PMID: 29727795 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical