Learn more: PMC Disclaimer | PMC Copyright Notice
Galactocerebroside-phospholipid interactions in bilayer membranes.
Abstract
Differential scanning calorimetry (DSC) and x-ray diffraction have been used to study the interaction of hydrated N-palmitoylgalactosylsphingosine (NPGS) and dipalmitoylphosphatidylcholine (DPPC). For mixtures containing less than 23 mol% NPGS, complete miscibility of NPGS into hydrated DPPC bilayers is observed in both the bilayer gel and liquid-crystal phases. X-ray diffraction data demonstrate insignificant differences in the DPPC-bilayer gel-phase parameters on incorporation of up to 23 mol% NPGS. At greater than 23 mol% NPGS, additional high-temperature transitions occur, indicating phase separation of cerebroside. For these cerebroside concentrations, at 20 degrees C, x-ray diffraction shows two lamellar phases, hydrated DPPC-NPGS gel bilayers (d = 64 A) containing 23 mol% NPGS, and NPGS "crystal" bilayers (d = 55 A). On heating to temperatures greater than 45 degrees C, the mixed DPPC-NPGS bilayer phase undergoes chain melting, and on further increasing the temperature progressively more NPGS is incorporated into the liquid-crystal DPPC-NPGS bilayer phase. At temperatures greater than 82 degrees C (the transition temperature of hydrated NPGS), complete lipid miscibility is observed at all DPPC/NPGS molar ratios.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Rumsby MG. Organization and structure in central-nerve myelin. Biochem Soc Trans. 1978;6(2):448–462. [PubMed] [Google Scholar]
- Bologa-Sandru L, Zalc B, Herschkowitz N, Baumann N. Oligodendrocytes of jimpy mice express galactosylceramide: an immunofluorescence study on brain sections and dissociated brain cell cultures. Brain Res. 1981 Nov 30;225(2):425–430. [PubMed] [Google Scholar]
- Reiss-Husson F. Structure des phases liquide-cristallines de différents phospholipides, monoglycérides, sphingolipides, anhydres ou en présence d'eau. J Mol Biol. 1967 May 14;25(3):363–382. [PubMed] [Google Scholar]
- Larsson D, Karlsson DA. Molecular arrangements in glycosphingolipids. Chem Phys Lipids. 1972 Mar;8(2):152–179. [PubMed] [Google Scholar]
- Fernandez-Bermudez S, Loboda-Cacković J, Cacković H, Hosemann R. Structure of cerebrosides I. Phrenosine at 23 degrees C and 66 degrees C. Z Naturforsch C. 1977 May-Jun;32(5-6):362–374. [PubMed] [Google Scholar]
- Hosemann R, Loboda-Cacković J, Cacković H, Fernandez-Bermúdez S, Baltá-Calleja FJ. Structure of cerebrosides. II. Small angle X-ray diffraction study of cerasine. Z Naturforsch C. 1979 Dec;34(12):1121–1124. [PubMed] [Google Scholar]
- Ruocco MJ, Atkinson D, Small DM, Skarjune RP, Oldfield E, Shipley GG. X-ray diffraction and calorimetric study of anhydrous and hydrated N-palmitoylgalactosylsphingosine (cerebroside). Biochemistry. 1981 Oct 13;20(21):5957–5966. [PubMed] [Google Scholar]
- Clowes AW, Cherry RJ, Chapman D. Physical properties of lecithin-cerebroside bilayers. Biochim Biophys Acta. 1971 Oct 12;249(1):301–317. [PubMed] [Google Scholar]
- Bunow MR. Two gel states of cerebrosides. Calorimetric and Raman spectroscopic evidence. Biochim Biophys Acta. 1979 Sep 28;574(3):542–546. [PubMed] [Google Scholar]
- Freire E, Bach D, Correa-Freire M, Miller I, Barenholz Y. Calorimetric investigation of the complex phase behavior of glucocerebroside dispersions. Biochemistry. 1980 Aug 5;19(16):3662–3665. [PubMed] [Google Scholar]
- Curatolo W. Thermal behavior of fractionated and unfractionated bovine brain cerebrosides. Biochemistry. 1982 Apr 13;21(8):1761–1764. [PubMed] [Google Scholar]
- Ladbrooke BD, Jenkinson TJ, Kamat VB, Chapman D. Physical studies of myelin. I. Thermal analysis. Biochim Biophys Acta. 1968 Sep 2;164(1):101–109. [PubMed] [Google Scholar]
- Correa-Freire MC, Freire E, Barenholz Y, Biltonen RL, Thompson TE. Thermotropic behavior of monoglucocerebroside--dipalmitoylphosphatidylcholine multilamellar liposomes. Biochemistry. 1979 Feb 6;18(3):442–445. [PubMed] [Google Scholar]
- Bunow MR, Levin IW. Molecular conformations of cerebrosides in bilayers determined by Raman spectroscopy. Biophys J. 1980 Dec;32(3):1007–1021. [PMC free article] [PubMed] [Google Scholar]
- Skarjune R, Oldfield E. Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance investigation of deuterium-labelled N-hexadeconoylgalactosylceramides (cerebrosides). Biochim Biophys Acta. 1979 Sep 21;556(2):208–218. [PubMed] [Google Scholar]
- Tardieu A, Luzzati V, Reman FC. Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases. J Mol Biol. 1973 Apr 25;75(4):711–733. [PubMed] [Google Scholar]
- Levine YK, Bailey AI, Wilkins MH. Multilayers of phospholipid bimolecular leaflets. Nature. 1968 Nov 9;220(5167):577–578. [PubMed] [Google Scholar]
- McIntosh TJ. The effect of cholesterol on the structure of phosphatidylcholine bilayers. Biochim Biophys Acta. 1978 Oct 19;513(1):43–58. [PubMed] [Google Scholar]
- Skarjune R, Oldfield E. Physical studies of cell surface and cell membrane structure. Deuterium nuclear magnetic resonance studies of N-palmitoylglucosylceramide (cerebroside) head group structure. Biochemistry. 1982 Jun 22;21(13):3154–3160. [PubMed] [Google Scholar]
- Lee RE, Worthington CR, Glew RH. The bilayer nature of deposits occurring in Gaucher's disease. Arch Biochem Biophys. 1973 Nov;159(1):259–266. [PubMed] [Google Scholar]