Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jul;43(1):91-101.
doi: 10.1016/S0006-3495(83)84327-6.

Galactocerebroside-phospholipid interactions in bilayer membranes

M J Ruocco, G G Shipley, E Oldfield

Galactocerebroside-phospholipid interactions in bilayer membranes

M J Ruocco et al. Biophys J. 1983 Jul.

Abstract

Differential scanning calorimetry (DSC) and x-ray diffraction have been used to study the interaction of hydrated N-palmitoylgalactosylsphingosine (NPGS) and dipalmitoylphosphatidylcholine (DPPC). For mixtures containing less than 23 mol% NPGS, complete miscibility of NPGS into hydrated DPPC bilayers is observed in both the bilayer gel and liquid-crystal phases. X-ray diffraction data demonstrate insignificant differences in the DPPC-bilayer gel-phase parameters on incorporation of up to 23 mol% NPGS. At greater than 23 mol% NPGS, additional high-temperature transitions occur, indicating phase separation of cerebroside. For these cerebroside concentrations, at 20 degrees C, x-ray diffraction shows two lamellar phases, hydrated DPPC-NPGS gel bilayers (d = 64 A) containing 23 mol% NPGS, and NPGS "crystal" bilayers (d = 55 A). On heating to temperatures greater than 45 degrees C, the mixed DPPC-NPGS bilayer phase undergoes chain melting, and on further increasing the temperature progressively more NPGS is incorporated into the liquid-crystal DPPC-NPGS bilayer phase. At temperatures greater than 82 degrees C (the transition temperature of hydrated NPGS), complete lipid miscibility is observed at all DPPC/NPGS molar ratios.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1967 May 14;25(3):363-82 - PubMed
    1. Biochim Biophys Acta. 1968 Sep 2;164(1):101-9 - PubMed
    1. Nature. 1968 Nov 9;220(5167):577-8 - PubMed
    1. Biochim Biophys Acta. 1971 Oct 12;249(1):301-17 - PubMed
    1. Chem Phys Lipids. 1972 Mar;8(2):152-79 - PubMed

Publication types

LinkOut - more resources

-