Aging-induced proinflammatory shift in cytokine expression profile in coronary arteries
- PMID: 12709402
- DOI: 10.1096/fj.02-1049fje
Aging-induced proinflammatory shift in cytokine expression profile in coronary arteries
Abstract
The phenotypic and functional changes of coronary arteries with aging promote ischemic heart disease. We hypothesized that these alterations reflect an aging-induced proinflammatory shift in vascular regulatory mechanisms. Thus, in isolated coronary arteries of young (3-month-old) and aged (25-month-old) male Fischer 344 rats the expression of 96 cytokines, chemokines, and their receptors were screened by a cDNA-based microarray technique. In aged vessels expressions of tumor necrosis factor (TNF)-alpha (3.3x), interleukin (IL)-1beta (3.0x), IL-6 (2.9x), IL-6Ralpha (2.8x) and IL-17 (6.1x) genes were significantly increased over young vessels. Quantitative reverse transcriptase-polymerase chain reaction confirmed these results. Western blotting demonstrated that protein expressions of TNF-alpha, IL-1beta, IL-6, and IL-17 were also significantly increased in vessels of aged rats compared with those of young rats. Immunofluorescent double labeling showed that in aged vessels IL-1beta and IL-6 are predominantly localized in the endothelium, whereas TNF-alpha and IL-17 are localized in smooth muscle. Thus, a proinflammatory shift in the profile of vascular cytokine expression may contribute to the aging-induced phenotypic changes in coronary arteries, promoting the development of ischemic heart disease in the elderly.
Similar articles
-
Omega-3 fatty acid regulates inflammatory cytokine/mediator messenger RNA expression in Porphyromonas gingivalis-induced experimental periodontal disease.Oral Microbiol Immunol. 2007 Aug;22(4):232-9. doi: 10.1111/j.1399-302X.2007.00346.x. Oral Microbiol Immunol. 2007. PMID: 17600534
-
Diseased vein grafts express elevated inflammatory cytokine levels compared with atherosclerotic coronary arteries.Ann Thorac Surg. 2004 May;77(5):1575-9. doi: 10.1016/j.athoracsur.2003.10.107. Ann Thorac Surg. 2004. PMID: 15111145
-
Proinflammatory phenotype of coronary arteries promotes endothelial apoptosis in aging.Physiol Genomics. 2004 Mar 12;17(1):21-30. doi: 10.1152/physiolgenomics.00136.2003. Physiol Genomics. 2004. PMID: 15020720
-
Inflammatory cytokines stimulated C-reactive protein production by human coronary artery smooth muscle cells.Circulation. 2003 Oct 21;108(16):1930-2. doi: 10.1161/01.CIR.0000096055.62724.C5. Epub 2003 Oct 6. Circulation. 2003. PMID: 14530191
-
[ON THE PREVENTION OF PREMATURE AGING OF THE HEART].Med Monatsschr. 1963 Sep;17:547-52. Med Monatsschr. 1963. PMID: 14052140 Review. German. No abstract available.
Cited by
-
Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis.Aging (Albany NY). 2023 Nov 8;15(21):12497-12512. doi: 10.18632/aging.205193. Epub 2023 Nov 8. Aging (Albany NY). 2023. PMID: 37944258 Free PMC article.
-
New Dawn for Atherosclerosis: Vascular Endothelial Cell Senescence and Death.Int J Mol Sci. 2023 Oct 13;24(20):15160. doi: 10.3390/ijms242015160. Int J Mol Sci. 2023. PMID: 37894840 Free PMC article. Review.
-
Tuberculosis and COVID-19 in the elderly: factors driving a higher burden of disease.Front Immunol. 2023 Sep 27;14:1250198. doi: 10.3389/fimmu.2023.1250198. eCollection 2023. Front Immunol. 2023. PMID: 37841265 Free PMC article. Review.
-
Thymic function and survival at advance ages in nursing home residents from Southern Italy.Immun Ageing. 2023 Apr 10;20(1):16. doi: 10.1186/s12979-023-00340-0. Immun Ageing. 2023. PMID: 37038200 Free PMC article.
-
Research progress on the mechanism of aging of vascular endothelial cells and the intervention of traditional Chinese medicine: A review.Medicine (Baltimore). 2022 Dec 9;101(49):e32248. doi: 10.1097/MD.0000000000032248. Medicine (Baltimore). 2022. PMID: 36626478 Free PMC article. Review.
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical