Structural and functional aspects of the respiratory chain of synaptic and nonsynaptic mitochondria derived from selected brain regions
- PMID: 1646801
- DOI: 10.1007/BF00762227
Structural and functional aspects of the respiratory chain of synaptic and nonsynaptic mitochondria derived from selected brain regions
Abstract
Studies on brain mitochondria are complicated by the regional, cellular, and subcellular heterogeneity of the central nervous system. This study was performed using synaptic and nonsynaptic mitochondria obtained from cortex, hippocampus, and striatum of male Sprague-Dawley rats (3 months old). Ubiquinone content, detected by HPLC analysis, was about 1.5 nmol/mg protein with an approximate CoQ9/CoQ10 molecular ratio of 2:1. The activities of several respiratory chain complexes were also studied (succinate-cyt. c reductase, NADH-cyt. c reductase, succinate-DCIP, ubiquinol2-cyt. c reductase, and cytochrome oxidase), and generally found to be higher in mitochondria from cortex than from other regions. Study of the activities of some of these enzymes vs. 1/T (Arrhenius plots) showed a straight line with an activation energy between 7 and 10 kcal/mol in all the three areas considered. Only CoQ2H2-cyt. c reductase activity revealed a biphasic temperature dependence. Also anisotropy (as fluorescence polarization) of the hydrophobic probe DPH showed a deviation from linearity; the break points for both enzymatic activity and anisotropy were found at about 23-24 degrees C.
Similar articles
-
Ubiquinone (coenzyme q10) and mitochondria in oxidative stress of parkinson's disease.Biol Signals Recept. 2001 May-Aug;10(3-4):224-53. doi: 10.1159/000046889. Biol Signals Recept. 2001. PMID: 11351130 Review.
-
Arrhenius plots of membrane-bound enzymes of mitochondria and microsomes in the brain cortex of developing and old rats.Mech Ageing Dev. 1986 Jun;35(1):1-15. doi: 10.1016/0047-6374(86)90061-8. Mech Ageing Dev. 1986. PMID: 3736127
-
Enzyme activities in perikaryal and synaptic mitochondrial fractions from rat hippocampus during development.Mech Ageing Dev. 1989 Sep;49(3):211-25. doi: 10.1016/0047-6374(89)90072-9. Mech Ageing Dev. 1989. PMID: 2554073 Review.
-
Brain cytochrome oxidase activity of synaptic and nonsynaptic mitochondria during aging.Basic Appl Histochem. 1989;33(2):139-45. Basic Appl Histochem. 1989. PMID: 2547357
-
Sequential damage in mitochondrial complexes by peroxidative stress.Neurochem Res. 1991 Dec;16(12):1295-302. doi: 10.1007/BF00966660. Neurochem Res. 1991. PMID: 1664494
Cited by
-
Effects of Chronic Hypertension on the Energy Metabolism of Cerebral Cortex Mitochondria in Normotensive and in Spontaneously Hypertensive Rats During Aging.Neuromolecular Med. 2024 Feb 23;26(1):2. doi: 10.1007/s12017-023-08772-z. Neuromolecular Med. 2024. PMID: 38393429
-
Alternative mitochondrial electron transfer for the treatment of neurodegenerative diseases and cancers: Methylene blue connects the dots.Prog Neurobiol. 2017 Oct;157:273-291. doi: 10.1016/j.pneurobio.2015.10.005. Epub 2015 Nov 18. Prog Neurobiol. 2017. PMID: 26603930 Free PMC article. Review.
-
Presynaptic mitochondria in functionally different motor neurons exhibit similar affinities for Ca2+ but exert little influence as Ca2+ buffers at nerve firing rates in situ.J Neurosci. 2010 Feb 3;30(5):1869-81. doi: 10.1523/JNEUROSCI.4701-09.2010. J Neurosci. 2010. PMID: 20130196 Free PMC article.
-
Examination of the brain mitochondrial lipidome using shotgun lipidomics.Methods Mol Biol. 2009;579:3-18. doi: 10.1007/978-1-60761-322-0_1. Methods Mol Biol. 2009. PMID: 19763468 Free PMC article.
-
Effect of ageing and ischemia on enzymatic activities linked to Krebs' cycle, electron transfer chain, glutamate and aminoacids metabolism of free and intrasynaptic mitochondria of cerebral cortex.Neurochem Res. 2009 Dec;34(12):2102-16. doi: 10.1007/s11064-009-0004-y. Neurochem Res. 2009. PMID: 19495970