Alpha- and beta-adrenergic induction of the expression of the uncoupling protein thermogenin in brown adipocytes differentiated in culture
- PMID: 1697859
Alpha- and beta-adrenergic induction of the expression of the uncoupling protein thermogenin in brown adipocytes differentiated in culture
Abstract
In order to examine the control of expression of the gene coding for the brown fat specific uncoupling protein thermogenin (UCP), brown fat cells isolated as undifferentiated precursors from the interscapular brown adipose tissue of young mice were grown in culture. In these cells, it was possible by norepinephrine (NE) addition to induce specifically the expression of the UCP gene. The effect of NE was due to activation of transcription. The ability to express the UCP gene was maximal in cells around confluence; cell cultures younger or older than this showed a lower response. The response to NE showed a sharp optimum around 0.1 microM and was linear with time over the 4-h period studied. The presence of insulin or thyroid hormones facilitated the NE response. Pharmacological analysis of the adrenergic response indicated that UCP gene expression could be induced both via beta-receptors (probably beta 3) and via alpha 1-receptors; these effects were synergistic. It was concluded that it is possible to promote these precursor cells to advance to such a state of differentiation that they can demonstrate the selective feature of the brown fat cell, i.e. the ability to express UCP. The expression of the UCP gene is regulated via interacting adrenergic mechanisms.
Similar articles
-
The uncoupling protein, thermogenin.Int J Biochem Cell Biol. 1998 Jan;30(1):7-11. doi: 10.1016/s1357-2725(97)00065-4. Int J Biochem Cell Biol. 1998. PMID: 9597749 Review.
-
Characterization of the novel brown adipocyte cell line HIB 1B. Adrenergic pathways involved in regulation of uncoupling protein gene expression.J Cell Sci. 1994 Jan;107 ( Pt 1):313-9. doi: 10.1242/jcs.107.1.313. J Cell Sci. 1994. PMID: 8175918
-
alpha- and beta-adrenergic control of thermogenin mRNA expression in brown adipose tissue.Biosci Rep. 1986 Jul;6(7):621-31. doi: 10.1007/BF01114756. Biosci Rep. 1986. PMID: 3022838
-
Adenosine 3',5'-monophosphate and thyroid hormone control of uncoupling protein messenger ribonucleic acid in freshly dispersed brown adipocytes.Endocrinology. 1992 May;130(5):2625-33. doi: 10.1210/endo.130.5.1374009. Endocrinology. 1992. PMID: 1374009
-
Beta-adrenergic, cAMP-mediated stimulation of proliferation of brown fat cells in primary culture. Mediation via beta 1 but not via beta 3 adrenoceptors.J Biol Chem. 1992 Jan 25;267(3):2006-13. J Biol Chem. 1992. PMID: 1346138
Cited by
-
Sex differences in thermoregulation in mammals: Implications for energy homeostasis.Front Endocrinol (Lausanne). 2023 Mar 8;14:1093376. doi: 10.3389/fendo.2023.1093376. eCollection 2023. Front Endocrinol (Lausanne). 2023. PMID: 36967809 Free PMC article. Review.
-
Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases.Cells. 2019 Jul 30;8(8):795. doi: 10.3390/cells8080795. Cells. 2019. PMID: 31366145 Free PMC article. Review.
-
Pirt deficiency has subtle female-specific effects on energy and glucose metabolism in mice.Mol Metab. 2019 May;23:75-81. doi: 10.1016/j.molmet.2019.02.011. Epub 2019 Mar 7. Mol Metab. 2019. PMID: 30902502 Free PMC article.
-
Promotion of lipid storage rather than of thermogenic competence by fetal versus newborn calf serum in primary cultures of brown adipocytes.Adipocyte. 2018;7(3):166-179. doi: 10.1080/21623945.2018.1479578. Epub 2018 Jul 16. Adipocyte. 2018. PMID: 29912625 Free PMC article.
-
β-Adrenergic receptors control brown adipose UCP-1 tone and cold response without affecting its circadian rhythmicity.FASEB J. 2018 Oct;32(10):5640-5646. doi: 10.1096/fj.201800452R. Epub 2018 May 1. FASEB J. 2018. PMID: 29715048 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
