Redox properties of the adenoside triphosphate-sensitive K+ channel in brain mitochondria
- PMID: 18189325
- DOI: 10.1002/jnr.21614
Redox properties of the adenoside triphosphate-sensitive K+ channel in brain mitochondria
Abstract
Brain mitochondrial ATP-sensitive K+ channel (mitoK(ATP)) opening by diazoxide protects against ischemic damage and excitotoxic cell death. Here we studied the redox properties of brain mitoK(ATP) . MitoK(ATP) activation during excitotoxicity in cultured cerebellar granule neurons prevented the accumulation of reactive oxygen species (ROS) and cell death. Furthermore, mitoK(ATP) activation in isolated brain mitochondria significantly prevented H2O2 release by these organelles but did not change Ca2+ accumulation capacity. Interestingly, the activity of mitoK(ATP) was highly dependent on redox state. The thiol reductant mercaptopropionylglycine prevented mitoK(ATP) activity, whereas exogenous ROS activated the channel. In addition, the use of mitochondrial substrates that led to higher levels of endogenous mitochondrial ROS release closely correlated with enhanced K+ transport activity through mitoK(ATP). Altogether, our results indicate that brain mitoK(ATP) is a redox-sensitive channel that controls mitochondrial ROS release.
Similar articles
-
Mitochondrial ATP-sensitive K+ channels are redox-sensitive pathways that control reactive oxygen species production.Free Radic Biol Med. 2007 Apr 1;42(7):1039-48. doi: 10.1016/j.freeradbiomed.2007.01.001. Epub 2007 Jan 8. Free Radic Biol Med. 2007. PMID: 17349931
-
Effects of ATP-sensitive potassium channel activators diazoxide and BMS-191095 on membrane potential and reactive oxygen species production in isolated piglet mitochondria.Brain Res Bull. 2005 Jul 30;66(2):85-90. doi: 10.1016/j.brainresbull.2005.03.022. Brain Res Bull. 2005. PMID: 15982523
-
Diazoxide protects against methylmalonate-induced neuronal toxicity.Exp Neurol. 2006 Sep;201(1):165-71. doi: 10.1016/j.expneurol.2006.04.004. Epub 2006 Jun 5. Exp Neurol. 2006. PMID: 16740260
-
The mitochondrial potassium cycle.IUBMB Life. 2001 Sep-Nov;52(3-5):153-8. doi: 10.1080/15216540152845948. IUBMB Life. 2001. PMID: 11798027 Review.
-
Mitochondrial K(ATP) channels in cell survival and death.J Mol Cell Cardiol. 2005 Jul;39(1):7-16. doi: 10.1016/j.yjmcc.2004.12.003. Epub 2005 Feb 19. J Mol Cell Cardiol. 2005. PMID: 15978901 Free PMC article. Review.
Cited by
-
Redox Regulation of Mitochondrial Potassium Channels Activity.Antioxidants (Basel). 2024 Apr 3;13(4):434. doi: 10.3390/antiox13040434. Antioxidants (Basel). 2024. PMID: 38671882 Free PMC article. Review.
-
Research progress of mitophagy in chronic cerebral ischemia.Front Aging Neurosci. 2023 Aug 3;15:1224633. doi: 10.3389/fnagi.2023.1224633. eCollection 2023. Front Aging Neurosci. 2023. PMID: 37600521 Free PMC article. Review.
-
The Interplay between Dysregulated Ion Transport and Mitochondrial Architecture as a Dangerous Liaison in Cancer.Int J Mol Sci. 2021 May 14;22(10):5209. doi: 10.3390/ijms22105209. Int J Mol Sci. 2021. PMID: 34069047 Free PMC article. Review.
-
Insulin actions on hypothalamic glucose-sensing neurones.J Neuroendocrinol. 2021 Apr;33(4):e12937. doi: 10.1111/jne.12937. Epub 2021 Jan 28. J Neuroendocrinol. 2021. PMID: 33507001 Free PMC article. Review.
-
Mitochondria, Bioenergetics and Excitotoxicity: New Therapeutic Targets in Perinatal Brain Injury.Front Cell Neurosci. 2017 Jul 12;11:199. doi: 10.3389/fncel.2017.00199. eCollection 2017. Front Cell Neurosci. 2017. PMID: 28747873 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
