Prodrug of green tea epigallocatechin-3-gallate (Pro-EGCG) as a potent anti-angiogenesis agent for endometriosis in mice
- PMID: 22948799
- DOI: 10.1007/s10456-012-9299-4
Prodrug of green tea epigallocatechin-3-gallate (Pro-EGCG) as a potent anti-angiogenesis agent for endometriosis in mice
Abstract
Green tea epigallocatechin-3-gallate (EGCG) can inhibit angiogenesis and development of an experimental endometriosis model in mice, but it suffers from poor bioavailability. A prodrug of EGCG (pro-EGCG, EGCG octaacetate) is utilized to enhance the stability and bioavailability of EGCG in vivo. In this study, the potential of pro-EGCG as a potent anti-angiogenesis agent for endometriosis in mice was investigated. Homologous endometrium was subcutaneously transplanted into mice to receive either saline, vitamin E, EGCG or pro-EGCG treatment for 4 weeks. The growth of the endometrial implants were monitored by IVIS(®) non-invasive in vivo imaging during the interventions. Angiogenesis of the endometriotic lesions was determined by Cellvizio(®) in vivo imaging and SCANCO(®) Microfil microtomography. The bioavailability, anti-oxidation and anti-angiogenesis capacities of the treatments were measured in plasma and lesions. The implants with adjacent outer subcutaneous and inner abdominal muscle layers were collected for histological, microvessel and apoptosis examinations. The result showed that EGCG and pro-EGCG significantly decreased the growth of endometrial implants from the 2nd week to the 4th week of intervention. EGCG and pro-EGCG significantly reduced the lesion size and weight, inhibited functional and structural microvessels in the lesions, and enhanced lesion apoptosis at the end of interventions. The inhibition by pro-EGCG in all the angiogenesis parameters was significantly greater than that by EGCG, and pro-EGCG also had better bioavailability and greater anti-oxidation and anti-angiogenesis capacities than EGCG. Ovarian follicles and uterine endometrial glands were not affected by either EGCG or pro-EGCG. Vitamin E had no effect on endometriosis. In conclusion, pro-EGCG significantly inhibited the development, growth and angiogenesis of experimental endometriosis in mice with high efficacy, bioavailability, anti-oxidation and anti-angiogenesis capacities. Pro-EGCG could be a potent anti-angiogenesis agent for endometriosis.
Similar articles
-
Therapeutic effects of green tea on endometriosis.Crit Rev Food Sci Nutr. 2023;63(18):3222-3235. doi: 10.1080/10408398.2021.1986465. Epub 2021 Oct 7. Crit Rev Food Sci Nutr. 2023. PMID: 34620005 Review.
-
Animal models for anti-angiogenic therapy in endometriosis.J Reprod Immunol. 2013 Mar;97(1):85-94. doi: 10.1016/j.jri.2012.10.012. J Reprod Immunol. 2013. PMID: 23432875 Review.
-
Natural therapies assessment for the treatment of endometriosis.Hum Reprod. 2013 Jan;28(1):178-88. doi: 10.1093/humrep/des369. Epub 2012 Oct 18. Hum Reprod. 2013. PMID: 23081870
-
Green tea epigallocatechin-3-gallate inhibits angiogenesis and suppresses vascular endothelial growth factor C/vascular endothelial growth factor receptor 2 expression and signaling in experimental endometriosis in vivo.Fertil Steril. 2011 Oct;96(4):1021-8. doi: 10.1016/j.fertnstert.2011.07.008. Epub 2011 Aug 6. Fertil Steril. 2011. PMID: 21821246
-
Anti-angiogenic effects of green tea catechin on an experimental endometriosis mouse model.Hum Reprod. 2009 Mar;24(3):608-18. doi: 10.1093/humrep/den417. Epub 2008 Dec 16. Hum Reprod. 2009. PMID: 19088106
Cited by
-
Epigallocatechin Gallate for the Treatment of Benign and Malignant Gynecological Diseases-Focus on Epigenetic Mechanisms.Nutrients. 2024 Feb 17;16(4):559. doi: 10.3390/nu16040559. Nutrients. 2024. PMID: 38398883 Free PMC article. Review.
-
Distinct molecular targets of ProEGCG from EGCG and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis.J Pharm Anal. 2024 Jan;14(1):100-114. doi: 10.1016/j.jpha.2023.09.005. Epub 2023 Sep 11. J Pharm Anal. 2024. PMID: 38352946 Free PMC article.
-
Diet in Prevention and Treatment of Endometriosis: Current State of Knowledge.Curr Nutr Rep. 2024 Mar;13(1):49-58. doi: 10.1007/s13668-024-00518-y. Epub 2024 Feb 7. Curr Nutr Rep. 2024. PMID: 38324218 Review.
-
Caffeic acid derivative WSY6 protects melanocytes from oxidative stress by reducing ROS production and MAPK activation.Heliyon. 2024 Jan 17;10(2):e24843. doi: 10.1016/j.heliyon.2024.e24843. eCollection 2024 Jan 30. Heliyon. 2024. PMID: 38304822 Free PMC article.
-
Phytochemicals Showing Antiangiogenic Effect in Pre-clinical Models and their Potential as an Alternative to Existing Therapeutics.Curr Top Med Chem. 2024;24(4):259-300. doi: 10.2174/0115680266264349231016094456. Curr Top Med Chem. 2024. PMID: 37867279 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical