Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
- PMID: 26517729
- DOI: 10.1016/j.ecoenv.2015.10.019
Evaluation of genetic damage in tobacco and arsenic exposed population of Southern Assam, India using buccal cytome assay and comet assay
Abstract
Ground water is the principal source of drinking water in Assam. Ground water contamination of arsenic in drinking water is a great concern for human health and considered as a human carcinogen. The present cytogenetic biomonitoring study was undertaken to investigate the genotoxic effects associated with people of southern Assam consuming arsenic contaminated water and chewing tobacco. Employing the buccal cytome assay, exfoliated cells were analyzed in 138 individuals of age range 22-42 years and divided into four groups. Group I (n=54) are participants residing in localities where ground water contains arsenic concentration below the permissible limit (<10μg/l) and without any tobacco chewing history. Group II (n=32) participants from the same area but they are tobacco chewers. Group III (n=24) participants from localities where significantly high arsenic contamination in ground water were observed. Whereas the Group IV (n=28) consists of participants from the arsenic contaminated area and also tobacco chewers. Body mass index (BMI) in all the groups are found to be nearly same and in normal range. Statistically significant (P<0.001) increase in genotoxic, cell death parameters and cell proliferation biomarkers were observed in the Group IV compared to other groups. In the comet assay, percent of tail DNA gradually increases among the groups and has statistical significance. Spearman correlation revealed strong positive correlation between the arsenic exposed peoples and the binucleated cells (r=0.4763; P<0.001). Amount of chewing tobacco had significant positive correlation with micronucleus frequency (r=0.268; P<0.05) and karyolitic cells (r=0.217; P<0.05) and also in the percentage of tail DNA (r=0.5532, P<0.001). A statistically significant increase in glucose content and decrease in hemoglobin content as well as acetylcholine esterase in the blood of exposed individuals was observed. Our preliminary study indicate that population exposed to arsenic through drinking water may become more susceptible towards chewing tobacco induced nuclear damage as evaluated by buccal cytome assay and comet assay.
Keywords: Acetylcholine esterase; Arsenic; Buccal cytome assay; Comet assay; Micronucleus; Tobacco.
Copyright © 2015 Elsevier Inc. All rights reserved.
Similar articles
-
Changes in buccal micronucleus cytome parameters associated with smokeless tobacco and pesticide exposure among female tea garden workers of Assam, India.Int J Hyg Environ Health. 2014 Mar;217(2-3):169-75. doi: 10.1016/j.ijheh.2013.04.007. Epub 2013 Apr 30. Int J Hyg Environ Health. 2014. PMID: 23706883
-
Cytotoxic and genotoxic effects in mechanics occupationally exposed to diesel engine exhaust.Ecotoxicol Environ Saf. 2019 Apr 30;171:264-273. doi: 10.1016/j.ecoenv.2018.12.067. Epub 2019 Jan 3. Ecotoxicol Environ Saf. 2019. PMID: 30612014
-
Micronucleus and other nuclear abnormalities among betel quid chewers with or without sadagura, a unique smokeless tobacco preparation, in a population from North-East India.Mutat Res. 2009 Jun-Jul;677(1-2):72-5. doi: 10.1016/j.mrgentox.2009.05.007. Epub 2009 May 23. Mutat Res. 2009. PMID: 19467343
-
Genetic toxicology of a paradoxical human carcinogen, arsenic: a review.Mutat Res. 2001 May;488(2):171-94. doi: 10.1016/s1383-5742(01)00056-4. Mutat Res. 2001. PMID: 11344043 Review.
-
The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay - an update and expanded photogallery.Mutat Res. 2013 Oct-Dec;753(2):100-113. doi: 10.1016/j.mrrev.2013.07.002. Epub 2013 Aug 11. Mutat Res. 2013. PMID: 23942275 Review.
Cited by
-
Update of the risk assessment of inorganic arsenic in food.EFSA J. 2024 Jan 18;22(1):e8488. doi: 10.2903/j.efsa.2024.8488. eCollection 2024 Jan. EFSA J. 2024. PMID: 38239496 Free PMC article.
-
Health Effects Associated With Pre- and Perinatal Exposure to Arsenic.Front Genet. 2021 Sep 29;12:664717. doi: 10.3389/fgene.2021.664717. eCollection 2021. Front Genet. 2021. PMID: 34659330 Free PMC article. Review.
-
Arsenic co-carcinogenesis: Inhibition of DNA repair and interaction with zinc finger proteins.Semin Cancer Biol. 2021 Nov;76:86-98. doi: 10.1016/j.semcancer.2021.05.009. Epub 2021 May 10. Semin Cancer Biol. 2021. PMID: 33984503 Free PMC article. Review.
-
The smokeless tobacco habit and DNA damage: A systematic review and meta-analysis.Med Oral Patol Oral Cir Bucal. 2019 Mar 1;24(2):e145-e155. doi: 10.4317/medoral.22846. Med Oral Patol Oral Cir Bucal. 2019. PMID: 30818306 Free PMC article.
-
Genetic damage in human populations at mining sites in the upper basin of the San Jorge River, Colombia.Environ Sci Pollut Res Int. 2019 Apr;26(11):10961-10971. doi: 10.1007/s11356-019-04527-1. Epub 2019 Feb 20. Environ Sci Pollut Res Int. 2019. PMID: 30788698
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical