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. 2016 Mar 21;21(3):377.
doi: 10.3390/molecules21030377.

Screening of Satureja subspicata Vis. Honey by HPLC-DAD, GC-FID/MS and UV/VIS: Prephenate Derivatives as Biomarkers

Igor Jerković  1 , Marina Kranjac  2 , Zvonimir Marijanović  3 , Marina Zekić  4 , Ani Radonić  5 , Carlo Ignazio Giovanni Tuberoso  6
Affiliations

Screening of Satureja subspicata Vis. Honey by HPLC-DAD, GC-FID/MS and UV/VIS: Prephenate Derivatives as Biomarkers

Igor Jerković et al. Molecules. .

Abstract

The samples of Satureja subspicata Vis. honey were confirmed to be unifloral by melissopalynological analysis with the characteristic pollen share from 36% to 71%. Bioprospecting of the samples was performed by HPLC-DAD, GC-FID/MS, and UV/VIS. Prephenate derivatives were shown to be dominant by the HPLC-DAD analysis, particularly phenylalanine (167.8 mg/kg) and methyl syringate (MSYR, 114.1 mg/kg), followed by tyrosine and benzoic acid. Higher amounts of MSYR (3-4 times) can be pointed out for distinguishing S. subspicata Vis. honey from other Satureja spp. honey types. GC-FID/MS analysis of ultrasonic solvent extracts of the samples revealed MSYR (46.68%, solvent pentane/Et2O 1:2 (v/v); 52.98%, solvent CH2Cl2) and minor abundance of other volatile prephenate derivatives, as well as higher aliphatic compounds characteristic of the comb environment. Two combined extracts (according to the solvents) of all samples were evaluated for their antioxidant properties by FRAP and DPPH assay; the combined extracts demonstrated higher activity (at lower concentrations) in comparison with the average honey sample. UV/VIS analysis of the samples was applied for determination of CIE Lab colour coordinates, total phenolics (425.38 mg GAE/kg), and antioxidant properties (4.26 mmol Fe(2+)/kg (FRAP assay) and 0.8 mmol TEAC/kg (DDPH assay)).

Keywords: GC-FID/MS; HPLC-DAD; Satureja subspicata Vis. honey; UV/Vis spectrometry; methyl syringate; prephenate derivatives.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The antiradical activity (a) and reducing potential (b) of S. subspicata Vis. honey extracts: EX1 (○) and EX2 (■).
See this image and copyright information in PMC

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