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. 2018 Apr 10;26(3):335-343.
doi: 10.3727/096504017X14907375885605. Epub 2017 Mar 28.

Long Noncoding RNA LINC01133 Functions as an miR-422a Sponge to Aggravate the Tumorigenesis of Human Osteosarcoma

Hai-Feng Zeng  1 , Hai-Yan Qiu  2 , Fa-Bo Feng  3
Affiliations

Long Noncoding RNA LINC01133 Functions as an miR-422a Sponge to Aggravate the Tumorigenesis of Human Osteosarcoma

Hai-Feng Zeng et al. Oncol Res. .

Retraction in

Abstract

Long noncoding RNAs (lncRNAs) have been verified to participate in various types of malignant tumors, including osteosarcoma (OS), which is the most common primary bone tumor with outstanding morbidity. Although an increasing number of lncRNAs have been reported to mediate the occurrence of OS, the potential mechanisms are still unclear. This study intends to uncover the mechanism by which lncRNA LINC01133 functions as an miRNA sponge to mediate OS tumorigenicity. In this study, we found that the expression level of LINC01133 was statistically upregulated in OS tumor tissue and cell lines compared to noncancerous tissues and a normal human osteoplastic cell line. LINC01133 silencing could also observably suppress the proliferation, migration, and invasion of OS cells (HOS and U2-OS). Bioinformatics analysis predicted that LINC01133 specifically targeted miR-422a, which was validated by dual-luciferase reporter assay. Furthermore, functional experiments revealed that miR-422a played a tumor-suppressive role in OS progression and could effectively reverse the function of LINC01133. In summary, our study discovered that lncRNA LINC01133 aggravates the proliferation, migration, and invasion of OS by sponging miR-422a, which provides a novel insight in the tumorigenesis of OS.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Overexpression of LINC01133 in osteosarcoma (OS) tissues and cell lines and its clinical prognosis. (A) Relative expression of LINC01133 examined by real-time quantitative polymerase chain reaction (qRT-PCR) in OS tissues and corresponding matched adjacent noncancerous tissues. (B) LINC01133 significantly overexpressed in OS cell lines (MG63, Saos-2, HOS, and U2-OS) compared to a normal human osteoplastic cell line (hFOB1.19). (C) Kaplan–Meier analysis and log-rank test were performed to assess the prognosis of OS patients. Data are presented as the mean ± standard deviation (SD). *p < 0.05, **p < 0.01 compared to hFOB1.19 or the noncancerous group.
Figure 2
Figure 2
Regulation of LINC01133 on the proliferation, migration, and invasion of OS cells. (A) LINC01133 silencing interfered with three types of oligonucleotides, and the interference efficiency is shown. (B) Cell viability was measured by the cell counting kit-8 (CCK-8) assay. (C) Colony formation assay observed after 48 h of transfection. (D) Migration ability of HOS and U2-OS cell lines detected by Transwell assay. (E) Invasion ability of HOS and U2-OS cell lines. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01 compared to the si-NC group.
Figure 3
Figure 3
LINC01133 functions as an miR-422a sponge in OS. (A) Expression levels of 21 predicted microRNAs (miRNAs) in LINC01133 silencing of the HOS cell line detected by qRT-PCR. (B) Putative complementary sites within miR-422a and 3′-UTR of LINC01133 predicted using starBase. (C) Dual-luciferase reporter assay verified the binding of miR-422a with LINC01133. (D) The expression level of miR-422a was confirmed in the HOS and U2-OS cell lines. (E) The expression level of miR-422a in OS tissues. (F) Pearson’s correlation analysis revealed the correlation between LINC01133 and miR-422a expression in OS tissues. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01 compared to the control group.
Figure 4
Figure 4
LINC01133 regulated OS proliferation, migration, and invasion by targeting miR-422a in the HOS cell line. (A) Expression of miR-422a was measured in multiple treated groups. (B) Cell proliferation viability of HOS cells was detected by the CCK-8 assay. (C) Cell colony formation assay. (D, E) Migration capacity was detected by the Transwell assays. (F, G) Invasion ability was detected by the Transwell assays. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01 compared to the control group.
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