Antrodia cinnamomea sensitizes radio-/chemo-therapy of cancer stem-like cells by modulating microRNA expression
- PMID: 28602756
- DOI: 10.1016/j.jep.2017.06.004
Antrodia cinnamomea sensitizes radio-/chemo-therapy of cancer stem-like cells by modulating microRNA expression
Abstract
Ethnopharmacological relevance: The discovery of many tissue-specific cancer stem cells (CSCs) continues to attract scientific attention. These CSCs are considered to be associated with chemo- and radio-resistance, and consequently, failure of conventional anticancer therapies. The recent demonstration of several microRNAs as enhancers of tumorigenicity via modulation of epithelial-mesenchymal transition and cancer stemness, makes them putative novel therapeutic target in oncology. Antrodia cinnamomea is a Chinese traditional medicine with several biological functions including anti-inflammation, antioxidant, and cancer prevention. However, the anti-CSC capability of A. Cinnamomea is not clear yet.
Aim of the study: To investigate the inhibitory effect of A. cinnamomea mycelium and extract on CSCs derived from various human cancer cell lines using our in-house therapeutics and human genome-wide miRNA screening panels.
Materials and methods: A broad range of human cancer cell lines, including the acute monocytic leukemia (THP-1), glioblastoma multiforme (GBM 8401), lung carcinoma (A549), breast adenocarcinoma (MDA-MB-231), hepatoblastoma (HepG2), colorectal adenocarcinoma (SW620), and foreskin fibroblast (HS68), were exposed to A. cinnamomea in this study. CD133+ CSCs generated from the cell lines were characterized and isolated by flow cytometry, effect of chemo- and radiotherapy was assessed using the MTT assay, while the RT-PCR and human genome wide qRT-PCR determined the differential gene expression patterns. A comparative analysis of the anticancer effect of A. cinnamomea and Cisplatin, Taxol, or irradiation was also performed.
Results: Our results indicated that A. cinnamomea mycelium and its ethyl acetate extracts showed anti-proliferation effects against all types of CSCs, especially the lung, breast, and head and neck squamous cell carcinoma CSCs. Furthermore, CSCs treatment with A. cinnamomea combined with irradiation or chemotherapeutics demonstrated significant anti-cancer effect. We also established an association between the CSC-inhibitory effect of A. cinnamomea and significant downregulation of several microRNAs and cancer stemness expression levels in brain and breast CSCs. More importantly, higher CD133 expression is associated with poor prognosis in glioblastoma and breast cancer patients.
Conclusion: Herein, we demonstrate the putative role of A. cinnamomea as an effective ethnopharmacologic therapeutic agent for cancer treatment.
Keywords: Antrodia cinnamomea; Cancer stem cell; Chemotherapy; Onco-microRNA; Radiotherapy.
Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.
Similar articles
-
Antrodia cinnamomea Enhances Chemo-Sensitivity of 5-FU and Suppresses Colon Tumorigenesis and Cancer Stemness via Up-Regulation of Tumor Suppressor miR-142-3p.Biomolecules. 2019 Jul 25;9(8):306. doi: 10.3390/biom9080306. Biomolecules. 2019. PMID: 31349708 Free PMC article.
-
Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway.BMC Complement Altern Med. 2018 May 9;18(1):152. doi: 10.1186/s12906-018-2204-y. BMC Complement Altern Med. 2018. PMID: 29743060 Free PMC article.
-
The Role of Cancer Stem Cells in Recurrent and Drug-Resistant Lung Cancer.Adv Exp Med Biol. 2016;890:57-74. doi: 10.1007/978-3-319-24932-2_4. Adv Exp Med Biol. 2016. PMID: 26703799 Review.
-
Global assessment of Antrodia cinnamomea-induced microRNA alterations in hepatocarcinoma cells.PLoS One. 2013 Dec 17;8(12):e82751. doi: 10.1371/journal.pone.0082751. eCollection 2013. PLoS One. 2013. PMID: 24358224 Free PMC article.
-
Epithelial-to-mesenchymal transition and cancer stem(-like) cells in head and neck squamous cell carcinoma.Cancer Lett. 2013 Sep 10;338(1):47-56. doi: 10.1016/j.canlet.2012.06.013. Epub 2012 Jul 4. Cancer Lett. 2013. PMID: 22771535 Review.
Cited by
-
Comparative chemical profiling of leaf essential oils from Cinnamomum kanehirae and related species using steam distillation and solvent extraction: Implications for plant-based classification.Heliyon. 2024 May 3;10(9):e30628. doi: 10.1016/j.heliyon.2024.e30628. eCollection 2024 May 15. Heliyon. 2024. PMID: 38726167 Free PMC article.
-
Roles of conventional and complementary therapies in recurrent hepatocellular carcinoma.World J Gastrointest Oncol. 2023 Jan 15;15(1):19-35. doi: 10.4251/wjgo.v15.i1.19. World J Gastrointest Oncol. 2023. PMID: 36684056 Free PMC article. Review.
-
Radiation therapy for triple-negative breast cancer: emerging role of microRNAs as biomarkers and radiosensitivity modifiers. A systematic review.Breast Cancer Res Treat. 2022 Jun;193(2):265-279. doi: 10.1007/s10549-022-06533-3. Epub 2022 Apr 9. Breast Cancer Res Treat. 2022. PMID: 35397079 Review.
-
Preliminary study on the relationship among stem cell markers, drug resistance and PI3K signaling pathway in multiple myeloma (MM) cell.Transl Cancer Res. 2020 May;9(5):3385-3391. doi: 10.21037/tcr-19-2116. Transl Cancer Res. 2020. PMID: 35117704 Free PMC article.
-
Patient-Derived Tumor Chemosensitization of GKB202, an Antrodia Cinnamomea Mycelium-Derived Bioactive Compound.Molecules. 2021 Oct 4;26(19):6018. doi: 10.3390/molecules26196018. Molecules. 2021. PMID: 34641562 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous