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. 2024 May 22;25(11):5638.
doi: 10.3390/ijms25115638.

Evaluation of Polyphenols Synthesized in Mature Seeds of Common Bean (Phaseolus vulgaris L.) Advanced Mutant Lines

Teodora G Yaneva  1 , Wieslaw Wiczkowski  2 , Andrey S Marchev  3 , Dida Iserliyska  1 , Milen I Georgiev  3 , Nasya B Tomlekova  4
Affiliations

Evaluation of Polyphenols Synthesized in Mature Seeds of Common Bean (Phaseolus vulgaris L.) Advanced Mutant Lines

Teodora G Yaneva et al. Int J Mol Sci. .

Abstract

This study aimed to investigate the availability of flavonoids, anthocyanins, and phenolic acids in mutant bean seeds, focusing on M7 mutant lines, and their corresponding initial and local cultivars. HPLC-DAD-MS/MS and HPLC-MS/MS were used to analyze twenty-eight genotypes of common bean. The obtained results suggest that the mutations resulted in four newly synthesized anthocyanins in the mutant bean seeds, namely, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, pelargonidin 3-O-glucoside, and petunidin 3-O-glucoside, in 20 accessions with colored seed shapes out of the total of 28. Importantly, the initial cultivar with white seeds, as well as the mutant white seeds, did not contain anthocyanins. The mutant lines were classified into groups based on their colors as novel qualitative characteristics. Five phenolic acids were further quantified: ferulic, p-coumaric, caffeic, sinapic, and traces of chlorogenic acids. Flavonoids were represented by epicatechin, quercetin, and luteolin, and their concentrations in the mutant genotypes were several-fold superior compared to those of the initial cultivar. All mutant lines exhibited higher concentrations of phenolic acids and flavonoids. These findings contribute to the understanding of the genetics and biochemistry of phenolic accumulation and anthocyanin production in common bean seeds, which is relevant to health benefits and might have implications for common bean breeding programs and food security efforts.

Keywords: EMS-induced mutagenesis; HPLC-MS/MS; anthocyanins; common bean (Phaseolus vulgaris L.); seed color.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

Figures

Figure 1
Figure 1
Concentrations of anthocyanins in mutant bean accessions at 520 nm via HPLC–DAD–MS/MS. Data are expressed as mean ± standard deviation from three independent experiments.
Figure 2
Figure 2
Seed clusters based on coat color: (a) A picture of the mutant bean seeds in comparison to their initial variety “Evros”; (b) dendrogram resulting from hierarchical clustering of 25 mutant bean (Phaseolus vulgaris L.) genotypes, initial and local cultivars using the nearest neighbor’s method.
Figure 3
Figure 3
Images of the twenty-six mutant lines of common bean developed in the M7 advanced mutant generation, the corresponding initial cultivar “Evros”, and a local cultivar “Tangra”.
Figure 3
Figure 3
Images of the twenty-six mutant lines of common bean developed in the M7 advanced mutant generation, the corresponding initial cultivar “Evros”, and a local cultivar “Tangra”.
See this image and copyright information in PMC

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References

    1. De Ron A.M., Papa R., Bitocchi E., González A.M., Debouck D.G., Brick M.A., Fourie D., Marsolais F., Beaver J., Geffroy V., et al. Common Bean. In: De Ron A., editor. Grain Legumes. Handbook of Plant Breeding. Volume 10. Springer; New York, NY, USA: 2015. - DOI
    1. Harlen W.C., Jati I.R.A. Chapter 8—Antioxidant activity of anthocyanins in common legume grains. In: Watson R.R., Preedy V.R., Zibadi S., editors. Polyphenols: Mechanisms of Action in Human Health and Disease. 2nd ed. Academic Press; Cambridge, MA, USA: 2018. pp. 81–92.
    1. Mihaylova D., Dimitrova-Dimova M., Popova A. Dietary Phenolic Compounds—Wellbeing and Perspective Applications. Int. J. Mol. Sci. 2024;25:4769. doi: 10.3390/ijms25094769. - DOI - PMC - PubMed
    1. Tomlekova N., Sofkova-Bobcheva S., Sarsu F., Baudoin J.P. Genetic diversity of Bulgarian Phaseolus vulgaris L. based on phaseolin type and seed-coat colour. Bulg. J. Agric. Sci. 2016;22:447–451.
    1. Sofkova-Bobcheva S., Pantchev I., Kiryakov I., Chavdarov P., Muhovski Y., Sarsu F., Tomlekova N. Mutation Breeding, Genetic Diversity and Crop Adaptation to Climate Change. CAB International; Wallingford, UK: 2021. Induced mutagenesis for improvement of bean (Phaseolus vulgaris L.) production in Bulgaria; pp. 178–193.

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