A comparative histochemical and immunohistochemical study of aminergic, cholinergic and peptidergic innervation in rat, hamster, guinea pig, dog and human livers
- PMID: 9831365
- DOI: 10.1111/j.1600-0676.1998.tb00817.x
A comparative histochemical and immunohistochemical study of aminergic, cholinergic and peptidergic innervation in rat, hamster, guinea pig, dog and human livers
Abstract
Aims/background: The mammalian liver receives both sympathetic and parasympathetic nerves that contain aminergic, cholinergic and peptidergic components. The intrahepatic distribution of nerve fibers are highly species-dependent; and also, even within one species, there are notable variations. To reveal the pattern and type of hepatic innervation in different species, we examined the distribution and density of these nerve fibers.
Methods: The livers of rats, golden hamsters, guinea pigs, dogs and humans were used. Aminergic and peptidergic nerve fibers were identified by immunohistochemistry for tyrosine hydroxylase (TH), neuropeptide Y (NPY), substance P (SP), vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP), and galanin (GAL), and cholinergic fibers were identified by the acetylcholinesterase (AChE) neurohistochemistry method.
Results: AChE-, TH-, NPY-, CGRP-, VIP-, and SP-positive nerves were observed in the connective tissue of the portal region, and they were in close contact with hepatic arteries, portal veins and bile ducts in all five species. Within the parenchyma of guinea pig, dog and human livers, TH-, NPY- and SP-positive fibers were observed, but no AChE- and CGRP-positive fibers were observed. In rat and hamster livers, no parenchymal nerve fibers could be demonstrated, but CGRP-, NPY- and SP-positive fibers were observed in the border of periportal areas. The density of CGRP-positive nerve fibers were slightly higher around bile ducts than around hepatic arteries and portal veins. GAL-positive fibers were not detected in any animal.
Conclusions: These data indicate that there were differences in the patterns of hepatic innervation among rats, golden hamsters, guinea pigs, dogs and humans. The data also show that: 1) in rat and hamster livers, hepatic functions may be regulated by both sympathetic and parasympathetic nerves in the portal region; 2) in guinea pig, dog and human livers they may be regulated by these fibers both in the interlobular region (parasympathetic and sympathetic systems) and in the intraparenchymal region (sympathetic system); and thus, 3) in the latter three species, hepatocytes and sinusoidal cells may be innervated by sympathetic nerves.
Similar articles
-
Anatomy of efferent hepatic nerves.Anat Rec A Discov Mol Cell Evol Biol. 2004 Sep;280(1):821-6. doi: 10.1002/ar.a.20087. Anat Rec A Discov Mol Cell Evol Biol. 2004. PMID: 15382019 Review.
-
Development of neuropeptide Y innervation in the liver.Microsc Res Tech. 1997 Nov 15;39(4):365-71. doi: 10.1002/(SICI)1097-0029(19971115)39:4<365::AID-JEMT6>3.0.CO;2-G. Microsc Res Tech. 1997. PMID: 9407546 Review.
-
Innervation of myofibroblast-like scleral spur cells in human monkey eyes.Invest Ophthalmol Vis Sci. 1995 Jul;36(8):1633-44. Invest Ophthalmol Vis Sci. 1995. PMID: 7601644
-
[Peptidergic innervation in the sinus hair follicles of several mammalian species].Kaibogaku Zasshi. 1992 Oct;67(5):623-33. Kaibogaku Zasshi. 1992. PMID: 1462755 Japanese.
-
Immunohistochemical and enzymehistochemical studies of peptidergic, aminergic and cholinergic innervation of the lacrimal gland of the monkey (Macaca fuscata).J Auton Nerv Syst. 1992 Mar;37(3):207-14. doi: 10.1016/0165-1838(92)90042-f. J Auton Nerv Syst. 1992. PMID: 1375236
Cited by
-
Hepatic interoception in health and disease.Auton Neurosci. 2024 Jun;253:103174. doi: 10.1016/j.autneu.2024.103174. Epub 2024 Mar 29. Auton Neurosci. 2024. PMID: 38579493 Review.
-
Role of cholinergic innervation in biliary remnants of patients with biliary atresia.Front Pediatr. 2024 Jan 8;11:1278978. doi: 10.3389/fped.2023.1278978. eCollection 2023. Front Pediatr. 2024. PMID: 38259596 Free PMC article.
-
Tumor-specific cholinergic CD4+ T lymphocytes guide immunosurveillance of hepatocellular carcinoma.Nat Cancer. 2023 Oct;4(10):1437-1454. doi: 10.1038/s43018-023-00624-w. Epub 2023 Aug 28. Nat Cancer. 2023. PMID: 37640929 Free PMC article.
-
Hepatic Innervations and Nonalcoholic Fatty Liver Disease.Semin Liver Dis. 2023 May;43(2):149-162. doi: 10.1055/s-0043-57237. Epub 2023 May 8. Semin Liver Dis. 2023. PMID: 37156523 Free PMC article. Review.
-
The Role of Catecholamines in Pathophysiological Liver Processes.Cells. 2022 Mar 17;11(6):1021. doi: 10.3390/cells11061021. Cells. 2022. PMID: 35326472 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous