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Status |
Public on Nov 04, 2014 |
Title |
Gene expression in macrophages derived form wild-type and Ndfst1-deficient mice before and after foam cell conversion |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
In order to examine the role of macrophage heparan sulfate proteoglycans in atherogenesis, we inactivated the biosynthetic gene N-acetylglucosamine N-deacetylase-N-sulfotransferase 1 (Ndst1) in macrophages. In order to determine the impact of altering Ndst1 expression, we crossbred mice bearing a conditional loxP-flanked (“floxed”) allele of Ndst1 (Ndst1f/f) to transgenic mice expressing the bacterial Cre recombinase under control of the lysozyme 2 promoter (LysMCre) to drive inactivation of the gene in myeloid cells. We compared the transcriptome of bone marrow derived macrophages from Ndst1f/fLysMCre- and Ndst1f/fLysMCre+ macrophages in basal growth medium and after foam cell conversion using 50µg/ml of aggregated LDL (agLDL) to asses the impact of altered macrophage heparan sulfate sulfation on gene expression.
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Overall design |
Total RNA obtained from bone marrow derived macrophages (BMDM) derived form two wild-type and two Ndfst1-deficient mice before and after foam cell conversion and analyzed in diplicate (n=4 for each conditions; total of 4 conditions)
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Contributor(s) |
Gordts PL, Erbilgin A, Lusis AJ, Esko JD |
Citation(s) |
25440058 |
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Submission date |
Sep 19, 2013 |
Last update date |
Jun 14, 2018 |
Contact name |
Philip Leo Stefan Maria Gordts |
E-mail(s) |
pgordts@ucsd.edu
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Organization name |
UCSD
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Department |
CMM
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Lab |
Esko Lab
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Street address |
9500 Gilman Dr #0687
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City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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Samples (16)
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Relations |
BioProject |
PRJNA219513 |