|
Status |
Public on Nov 04, 2014 |
Title |
WT_2.1 |
Sample type |
RNA |
|
|
Source name |
BMDM, Ndfst1 wild type, 0 µg/ml agLDL
|
Organism |
Mus musculus |
Characteristics |
background strain: C57BL6 age: 12 week old cell type: bone marrow derived macrophages phenotype: Ndfst1 wild type
|
Treatment protocol |
BMDM were treated with or without 50 µg/ml of aggregated LDL for 24 hours in DMEM/F12 medium supplemented with 10% lipoprotein-eficient fetal calf serum, 100 units/ml penicillin, 100 μg/ml streptomycin and 20 ng/ml recombinant mouse Macrophage Colony Stimulatinf Factor 1
|
Growth protocol |
BMDM were grown in DMEM/F12 medium supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 μg/ml streptomycin and 20 ng/ml recombinant mouse Macrophage Colony Stimulatinf Factor 1
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
standard Illumina
|
|
|
Hybridization protocol |
standard Illumina
|
Scan protocol |
standard Illumina
|
Description |
Ndst1f/fLysMCre- mouse 2 Replicate 1 6668263002_A
|
Data processing |
Illumina GenomeStudio: cubic spline normalization
|
|
|
Submission date |
Sep 19, 2013 |
Last update date |
Nov 04, 2014 |
Contact name |
Philip Leo Stefan Maria Gordts |
E-mail(s) |
pgordts@ucsd.edu
|
Organization name |
UCSD
|
Department |
CMM
|
Lab |
Esko Lab
|
Street address |
9500 Gilman Dr #0687
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE51009 |
Gene expression in macrophages derived form wild-type and Ndfst1-deficient mice before and after foam cell conversion |
|