|
Status |
Public on Sep 05, 2018 |
Title |
6h_1 |
Sample type |
SRA |
|
|
Source name |
interscapular brown adipose tissue
|
Organism |
Mus musculus |
Characteristics |
tissue: interscapular brown adipose tissue ambient temperature: 5°C duration of cold exposure: 6 hours strain: C57BL/6J gender: male
|
Extracted molecule |
total RNA |
Extraction protocol |
Interscapular BAT was excised and flash frozen. Total RNA was prepared from frozen tissue with Trisue (Bioline) and column-purified (SV Total RNA Isolation System, Promega). Libraries were constructed from total RNA (TruSeq RNA Sample Prep kit v2 (Illumina)).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Sequencing software: HiSeq Control Software 2.2.58 Image analysis and base calling: Real-Time Analysis (RTA) 1.18.64 fastq-file generation: CASAVA BCL2FASTQ Conversion Software 1.8.3 Sequenced reads with masked adaptor sequence were mapped to the murine genome, NCBI build 38, by the Genomatix Mapper 3.7.6 (Genomatix Mining Station, settings: 'deep', min. quality 92%). Unique hits were subjected to expression analysis for RNA-seq (Genomatix Genome Analyzer, ElDorado version 12-2013) and provided as reads per kilobase of exon per megabase of library size (RPKM). Genome_build: murine NCBI genome build 38 Supplementary_files_format_and_content: tab-delimited text file providing RPKM values for each sample.
|
|
|
Submission date |
Sep 04, 2018 |
Last update date |
Sep 05, 2018 |
Contact name |
Tobias Fromme |
E-mail(s) |
fromme@tum.de
|
Organization name |
Technical University of Munich
|
Department |
Molecular Nutritional Medicine
|
Street address |
Gregor-Mendel-Str. 2
|
City |
Freising |
ZIP/Postal code |
85354 |
Country |
Germany |
|
|
Platform ID |
GPL17021 |
Series (1) |
GSE119452 |
Transcriptome alterations of murine brown adipose tissue during cold exposure |
|
Relations |
BioSample |
SAMN09953920 |
SRA |
SRX4639157 |