|
| Status |
Public on Aug 04, 2022 |
| Title |
Wildtype cortex P7 mouse #2 [Sample 2] |
| Sample type |
SRA |
| |
|
| Source name |
cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6J
genotype: WT
developmental stage: PND7
Sex: male
tissue: cortex
|
| Growth protocol |
Cortices and hippocampi were disected from 5 wildtype and 5 Ube3aOE P7 pups
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen).
RNA libraries for RNA-seq were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence
Trimmed sequence reads were mapped to GrCM38/mm10 using HiSat2 (version 2.1.0)
We called gene expression values using htseq-count(version 0.11.2)
Assembly: mm10
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
May 31, 2022 |
| Last update date |
Aug 04, 2022 |
| Contact name |
Mattijs Punt |
| E-mail(s) |
a.m.punt@erasmusmc.nl
|
| Phone |
0648216382
|
| Organization name |
Erasmus MC
|
| Department |
Clinical Genetics
|
| Street address |
Dr. Molewaterplein 40
|
| City |
Rotterdam |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
3015 GD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE205128 |
Molecular and behavioral consequences of Ube3a gene overdosage in mice |
|
| Relations |
| BioSample |
SAMN28772898 |
| SRA |
SRX15503244 |
