Serum selenium concentration is associated with metabolic factors in the elderly: a cross-sectional study

Abstract

Background

Selenium, an essential trace element, is vital for human normal development, growth, male fertility, and thyroid hormone metabolism. Selenium deficiency is associated with Keshan disease, an endemic cardiomyopathy, and Kashin-Beck disease, a deforming arthritis. Less-overt deficiency may cause disease susceptibility and poor health maintenance [1]. Selenoproteins reportedly possess antioxidant properties and its deficiency plays an important role in the pathogenesis of cardiovascular disease (CVD). However, whether or not selenium concentration contributes to CVD remains uncertain. For example, epidemiologic studies show that low serum selenium is associated with increased cardiovascular mortality [2-4]. On the other hand, there is no significant correlation between selenium and risk of myocardial infarction [5] and peripheral artery disease [6] in selenium-replete population such as United States.

Some studies propose that selenium may exert its influence on CVD through its effect on known CVD risk factors, including dyslipidemia and hyperglycemia. Observational studies show positive associations of serum selenium with total cholesterol, low-density lipoprotein (LDL) cholesterol, and triglycerides concentrations in high selenium-replete populations [7-9]. However, in populations with low serum selenium concentrations, the associations are inconsistent [10-19]. Similarly inconsistent is the association between serum selenium and fasting glucose concentrations [7,20-22]. Potential adverse effects of high selenium exposure on diabetes risk have been noted [23-26]. Nonetheless, most studies involve relatively young populations. Few studies have focused on the elderly.

Since CVD is a leading cause of death in the elderly, the relationship between serum selenium concentrations and metabolic factors deserves further studies. This study aimed to evaluate the relationship between serum selenium and metabolic factors, including serum lipid and glucose concentrations, in a cohort of Taiwanese elderly.

Methods

Results

The basic characteristics of the subjects were shown in Table ​Table1.1. Their average age was 71.5 ± 4.6 years and mean serum selenium concentration was 1.14 ± 0.23 μmol/L. Serum total, LDL and HDL cholesterol were significantly higher in women (P < 0.001, P = 0.016 and P < 0.001, respectively) than in men but there was no significant difference in their selenium levels. However, fasting glucose was significantly higher in men than in women (P = 0.036). In Table ​Table1,1, the means of total, LDL cholesterol, triglycerides, and fasting glucose concentrations were significantly different among the four selenium quartiles (test for trend, P = 0.0004, P = 0.02, P = 0.0008 and P = 0.04, respectively). In sex specific analyses, the means of triglycerides and fasting glucose concentrations increased significantly across the four selenium quartiles in men (P = 0.01 and P = 0.001, respectively) while the means of total, HDL and LDL cholesterol concentrations increased significantly across the four selenium quartiles (P < 0.0001, P = 0.028 and P < 0.0001, respectively) in women. The characteristics among the four selenium quartiles separately for men and women were shown in additional file 1, Table S1 and Table S2, respectively.

Serum selenium concentration was positively associated with serum total, LDL cholesterol, and triglycerides using multivariate linear regression analyses adjusting for various confounders in Models 1-3 in Table ​Table2.2. Serum fasting glucose was also positively associated with serum selenium in Model 1, Model 2 and "modified" Model 3 (P = 0.026, P = 0.034 and P = 0.03, respectively). The result of sex-specific analyses was provided in additional file 1, Table S3 and Table S4.

The LS means of total, LDL, HDL cholesterol, and triglycerides in the four selenium quartiles were shown in Figure ​Figure1.1. The LS means of total (Figure ​(Figure1A),1A), LDL cholesterol (Figure ​(Figure1B),1B), and triglycerides (Figure ​(Figure1D)1D) increased significantly with increments of serum selenium quartiles. However, no significant difference was found in the LS means of HDL cholesterol (Figure ​(Figure1C)1C) with increments of serum selenium.

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Figure 1

Relationship between serum selenium and total (A), LDL (B), HDL cholesterol (C), and triglycerides (D) levels. The least square (LS) means of serum lipid in the quartiles of serum selenium (<0.98 μmol/L, 0.98-1.136 μmol/L, 1.14-1.3 μmol/L, >1.3 μmol/L in serum selenium) using general linear model after adjustment for confounders in model 3. Covariates appearing in the models were estimated with the following values: age = 71.4968, sex = 0.69, BMI = 23.423, current smoking = 0.065, current drinking = 0.145, vegetarian diet = 0.03, physical activity = 332.85, hormone replacement therapy = 0.18, cholesterol-lowering medication = 0.21, vitamin supplement = 0.77, diabetes mellitus = 0.12, hypertension = 0.465. The LS means of total cholesterol increased with the increments of serum selenium (P for trend < 0.001); the LS means of log transformation of triglycerides increased with increments of serum selenium (P for trend < 0.01); the LS means of LDL cholesterol increased with increments of serum selenium (P for trend < 0.001), and the LS means of HDL cholesterol increased with increments of serum selenium (P for trend = 0.152).

Discussion

In this study, serum selenium concentrations were positively associated with serum concentrations of total cholesterol, LDL cholesterol, triglycerides, and glucose in the elderly, after adjustment for age, gender, anthropometric indices, lifestyles, and traditional CVD risk factors. This suggests that high serum selenium concentrations may be associated with risk factors of CVD in the elderly.

Positive relationships between serum selenium and total cholesterol concentrations are discussed in several studies of various serum selenium concentrations [2,4,7,10-15,19,27]. However, most participants have been young and middle-aged adults. Similar to two studies involving the elderly [4,14,15], this study showed that serum total cholesterol concentration was positively associated with serum selenium concentration (Table ​(Table2;2; Fig. ​Fig.1A1A).

Many studies failed to show a significant association between serum selenium and triglycerides concentrations [10,12,27] and only two have shown a positive association [7,8]. Our study demonstrated that serum selenium concentrations were positively associated with serum triglycerides (Table ​(Table2;2; Fig. ​Fig.1D1D).

The association between serum selenium and LDL cholesterol levels was not consistent in previous studies. Five studies showed a positive association [7-9,13,14] but others revealed no significant relationship [11,16-18]. The variable sample sizes, age groups, and lack of adjustments for possible confounders may account for the inconsistencies in those studies. Our present study supported that serum LDL cholesterol concentrations were positively associated with serum selenium levels (Table ​(Table2;2; Fig. ​Fig.1B1B).

The association of serum selenium with HDL cholesterol is not consistent in previous studies. Five studies report a positive association [8,9,11,15,16], one has a negative association [7], and three do not show any significant association [10,12,19]. In our present study, the HDL cholesterol concentrations did not increase significantly with increments of serum selenium (Table ​(Table2;2; Fig. ​Fig.1C).1C). In addition, the mean HDL cholesterol concentration was higher in our population as compared to those in previous studies.

Interestingly, the effects of selenium supplementation on blood lipids are contradictory in animal and human studies. In rats, selenium supplementation increases LDL receptor activity [28,29] but decreases 3-hydroxy 3-methylglutaryl co-enzyme A (HMG-CoA) reductase expression [30], leading to decreased plasma LDL cholesterol and total cholesterol levels. However, one animal study in mice showed a significant increase in plasma cholesterol with the loss of housekeeping selenoprotein expression [31]. In human, selenium supplementation was found to increase total cholesterol and triglyceride levels in French adults [27]. Total cholesterol and LDL cholesterol levels also increased after selenium supplementation in the Chinese population [32]. Another study showed no further decrease in triglyceride or LDL cholesterol concentration but a blunted increment of HDL with selenium supplementation in participants with coronary heart disease receiving simvastatin-niacin treatment [33]. Therefore, the role of selenium supplementation on lipid metabolism in humans deserves further research. Recently, the apoE δ4 gene was found to play a central role between selenium levels and lipid metabolism in rural elderly Chinese [34]. The underlying interactive mechanism between susceptible gene, selenium, and lipids needs further investigation.

High serum selenium concentrations also correlate with high fasting glucose concentrations [7,20]. The prevalence of type 2 diabetes increases with increment of serum selenium in American adults [23]. Further analysis of the study shows that glycosylated hemoglobin levels increase with increasing selenium concentrations [26]. Similarly, serum selenium level was positively associated with glucose level in Table ​Table2.2. Stranges et al note increased risk of diabetes after receiving selenium supplements for 7.7 years [24]. Lipman et al also found a statistically non-significant increase in diabetes after selenium supplementation for 5.46 years [25]. Interestingly, over-production of selenium-dependent glutathione peroxidase-1 can induce hyperinsulinemia in mice [35]. In contrast, Hughes et al showed no significant difference in serum selenium levels between diabetic and non-diabetic Singaporeans [36]. Recently, Akbaraly et al found that higher serum selenium status at baseline had protective effect on later occurrence of dysglycemia in a 9-year longitudinal study [22]. Besides, supplementation of selenium can lead to increased insulin sensitivity in rats [37]. Therefore, the inconsistent results of various studies imply that further research is warranted.

Although this study shows that elevated serum selenium concentrations are associated with metabolic factors in an elderly population, it has some limitations. First, the study has a cross-sectional design and causal inference cannot be established. Second, the participants are volunteers rather than randomly selected. External validation is necessary in future studies. In addition, there is no human selenium survey in Taiwan. Thus, a comparison of serum selenium concentrations between the data here and the Taiwanese data is not possible. Thirdly, there is no available data on food intake and selenium supplementation, which may indicate a possible residual confounding effect. Fourth, multivariate analyses adjusted for lipid-lowering medications might bias the results [38]. Finally, only the serum fasting glucose without insulin levels was available in the study. Therefore, the relationship between selenium with insulin resistance could not be explored.

Conclusions

In conclusion, serum selenium concentration was associated with serum concentrations of total, LDL cholesterol, triglycerides, and glucose in the elderly after adjustment for potential confounders. The role of selenium on lipid and glucose metabolism in humans deserves further research in the future. Thus, recommendations on selenium supplementation should be carefully evaluated.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

KCY was in charge of data analysis and writing of the draft. LTL, CYC participated in the data collection and study design. HYH was responsible for serum selenium measurement. YSL helped with the data management and interpretation of results. KCH synthesized the analyses and headed the writing of the manuscript. All of the authors read and approved the final manuscript.

Supplementary Material

References