Figure 6

Let-7f-5p reverses Dex-induced bone loss in mice. (A) Increased let-7f-5p expression in bone following injection of let-7f-5p (agomir) using Invivofectamine 2.0 reagent into 6-week-old female C57 mice. Results are shown as mean ± s.d., n=6, ^*P < 0.05, ^**P < 0.01, and ^#P < 0.05 by ANOVA with Tukey's post hoc test. (B) micro-CT scanning of lumbar 4 vertebrae. (C-D) Histomorphometric analysis of the trabecular bone in vertebrae. Results are shown as mean ± s.d., n=6, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 by ANOVA with Tukey's post hoc test. (E) The serum levels of P1NP and OCN. Results are shown as mean ± s.d., n=6, ^*P < 0.05, ^**P < 0.01, and ^##P < 0.01 by ANOVA with Tukey's post hoc test.