Figure 2

Rut enhances thermogenic program and increases mitochondrial respiration of primary brown and beige adipocytes. (A) Expression of thermogenic genes in differentiated primary brown adipocytes (BAT-SVF). (B) UCP1 and PGC-1α protein expression level in BAT-SVF. (C) OCR in differentiated primary brown adipocytes on basal condition, in the presence of 2 μM oligomycin, 1 μM FCCP or 1 μM rotenone/antimycin. (D) Statistical graphs of basal and uncoupled respiration in BAT-SVF. (E) Expression of thermogenic and fuel consuming genes in functional primary subcutaneous adipocytes (Sub-SVF). (F) Western blot analysis of PGC-1α, UCP1 protein levels in Sub-SVF. (G) The testing of OCR in Rut-treated Sub-SVF. (H) Statistical values of basal and uncoupled respiration in Sub-SVF. n = 3 per group. Data are presented as the means ±SEM. * p < 0.05, ** p < 0.01, Rut groups versus vehicle group by one-way ANOVA.