Figure 3

Rut restrains the weight gain of HFD-fed mice and ameliorates insulin resistance. (A) Body weights were recorded each week after Rut treatment. (B) Tissues were collected and weighted. (C) Proportion of body composition of mice. (D) Representative hematoxylin and eosin staining (HE staining) from interscapular BAT and inguinal WAT sections of HFD-fed mice after 8 weeks of Rut treatment. Scale bar, 50 µm. (E,F) Quantification of adipocyte area of iBAT (E) and iWAT (F). (G) Blood glucose curves during testing of oral glucose tolerance (GTT). (H) Area under curve (AUC) of GTT. (I) Blood glucose levels were plotted in insulin tolerance test (ITT). (J) Analysis of AUC during ITT. n = 6 per group. Data are presented as the means ±SEM. Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the indicated control group.