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. 2022 Nov 13;14(22):4805.
doi: 10.3390/nu14224805.

The Effect of the Restrictive Ketogenic Diet on the Body Composition, Haematological and Biochemical Parameters, Oxidative Stress and Advanced Glycation End-Products in Young Wistar Rats with Diet-Induced Obesity

Natalia Drabińska  1 , Jerzy Juśkiewicz  2 , Wiesław Wiczkowski  1
Affiliations

The Effect of the Restrictive Ketogenic Diet on the Body Composition, Haematological and Biochemical Parameters, Oxidative Stress and Advanced Glycation End-Products in Young Wistar Rats with Diet-Induced Obesity

Natalia Drabińska et al. Nutrients. .

Abstract

Over the past few years, the interest in the application of the ketogenic diet (KD) for obesity management is growing. Although many studies have been performed on the effects of KD, the metabolic and physiological impact of KD is still not fully understood. Therefore, this study aimed to evaluate the effect of calorie-restricted KD on the body weight and composition, oxidative stress, and advanced glycation end products (AGEs) assessed in an animal model with young Wistar rats. KD was followed for 4 weeks in maturity after an obesity-inducing high-fat diet during adolescence, resulting in a slowing down of the weight gain but higher adiposity compared to a standard diet. Increased adiposity resulted in an deterioration of liver parameters, suggesting negative changes in this organ. No adverse effects of KD were determined in haematological parameters in young rats. KD did not affect AGEs; however, a decrease in oxidative stress was observed. Based on the presented results, it can be concluded that KD applied for weight loss in obesity induced in adolescence may reduce oxidative stress without compromising the haematological status; however, caution may be required to control adiposity, glucose level and liver health. Thus, KD therapy should be carefully controlled, especially in young subjects.

Keywords: advanced glycation end-products; ketogenic diet; low carbohydrate diet; obesity; oxidative stress.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The design of the animal study. HFD—high-fat diet; SD—standard diet; RKD—restrictive ketogenic diet; RSD—restrictive standard diet.
Figure 2
Figure 2
The changes in the body weight, weight gain, percentage of lean and fat mass during 4 weeks of KD or control diet. The p-values of the two-way ANOVA analysis are presented in Table.
Figure 3
Figure 3
The status of leptin before and after the nutritional intervention. The bars represents the median with the error bars representing interquartile range (Q1; Q3). The significant differences are marker with “*”.
Figure 4
Figure 4
The status of malonaldehyde and SOD before and after the nutritional intervention. The bars represents the median with the error bars representing interquartile range (Q1; Q3). The significant differences are marked with “*”.
Figure 5
Figure 5
The plasma concentration of advanced glycation end-products (AGEs) before and after the intervention. The bars represents the mean with the error bars representing standard deviation.
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