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DOI: 10.1055/s-0035-1556408
Temperature-dependent metabolite production by Arctic actinomycetes
Actinomycetes are a well-established source of natural products with therapeutic potential, particularly for the treatment of infectious diseases. The exploration of microbes from underexplored habitats and the application of novel cultivation conditions are proven strategies for the discovery of novel bioactive metabolites. We set out to explore the metabolic diversity of cold-adapted actinomycetes isolated from marine sediments collected from Canada's Arctic. To screen for metabolites differentially regulated by temperature, 45 strains were fermented at 15 °C and 30 °C and the resulting chemical extracts were tested for antimicrobial activity. Seventy-six percent of the strains exhibited antimicrobial activity against one or more pathogens. Strikingly, extracts prepared from fermentations incubated at 15 °C generated double the number of hits than fermentations conducted at 30 °C. Overall, the majority (44%) of strains exhibited a greater frequency of antimicrobial activity when fermented at 15 °C. Metabolomic analysis (LC-HRMS) was utilized to assess the differential production of metabolites in response to temperature. The results of these experiments will be presented.