Assessment of Brown and Beige Adipose Tissue Activation in Mice Using PET/CT Imaging
- PMID: 37076677
- DOI: 10.1007/978-1-0716-3167-6_12
Assessment of Brown and Beige Adipose Tissue Activation in Mice Using PET/CT Imaging
Abstract
In response to cold induction, brown adipose tissues (BAT) and emerged brown-like adipocytes (beige adipocytes) in subcutaneous white adipose tissues (WAT browning/beiging) are activated. Thermogenesis is increased during glucose and fatty acid uptake and metabolism in adult humans and mice. This activation of BAT or WAT beiging to generate heat helps to counteract diet-induced obesity. This protocol applies the glucose analog radiotracer 18F-fluorodeoxyglucose (FDG), coupled with positron emission tomography and computed tomography (PET/CT) scanning to evaluate cold-induced thermogenesis in the active BAT (interscapular region) and browned/beiged WAT (subcutaneous adipose region) in mice. The PET/CT scanning technique not only can quantify cold-induced glucose uptake in well-known BAT and beige-fat depots but also helps to visualize the anatomical location of novel uncharacterized mouse BAT and beige fat where cold-induced glucose uptake is high. Histological analysis is further employed to validate signals of delineated anatomical regions in PET/CT images as bona fide mouse BAT or beiged WAT fat depots.
Keywords: Beige adipose tissue; Brown adipose tissue; Cold-induced thermogenesis; PET/CT scintigraphy; Uncoupling protein 1 (UCP1).
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Similar articles
-
Many Ways to Rome: Exercise, Cold Exposure and Diet-Do They All Affect BAT Activation and WAT Browning in the Same Manner?Int J Mol Sci. 2022 Apr 26;23(9):4759. doi: 10.3390/ijms23094759. Int J Mol Sci. 2022. PMID: 35563150 Free PMC article. Review.
-
Role of Brown and Beige Adipose Tissues in Seasonal Adaptation in the Raccoon Dog (Nyctereutes procyonoides).Int J Mol Sci. 2021 Sep 6;22(17):9623. doi: 10.3390/ijms22179623. Int J Mol Sci. 2021. PMID: 34502532 Free PMC article.
-
Overexpression of Adiponectin Receptor 1 Inhibits Brown and Beige Adipose Tissue Activity in Mice.Int J Mol Sci. 2021 Jan 18;22(2):906. doi: 10.3390/ijms22020906. Int J Mol Sci. 2021. PMID: 33477525 Free PMC article.
-
Imaging adipose tissue browning using the TSPO-18kDa tracer [18F]FEPPA.Mol Metab. 2019 Jul;25:154-158. doi: 10.1016/j.molmet.2019.05.003. Epub 2019 May 6. Mol Metab. 2019. PMID: 31105057 Free PMC article.
-
Do estrogens enhance activation of brown and beiging of adipose tissues?Physiol Behav. 2018 Apr 1;187:24-31. doi: 10.1016/j.physbeh.2017.09.026. Epub 2017 Oct 6. Physiol Behav. 2018. PMID: 28988965 Review.
References
-
- Cypess AM, Lehman S, Williams G et al (2009) Identification and importance of Brown adipose tissue in adult humans. N Engl J Med 360:1509–1517. https://doi.org/10.1056/NEJMoa0810780 - DOI - PubMed - PMC
-
- Virtanen KA, Lidell ME, Orava J et al (2009) Functional Brown adipose tissue in healthy adults. N Engl J Med 360:1518–1525. https://doi.org/10.1056/NEJMoa0808949 - DOI - PubMed
-
- van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM et al (2009) Cold-activated Brown adipose tissue in healthy men. N Engl J Med 360:1500–1508. https://doi.org/10.1056/NEJMoa0808718 - DOI - PubMed
-
- Cypess AM (2022) Reassessing human adipose tissue. N Engl J Med 386:768–779. https://doi.org/10.1056/NEJMra2032804 - DOI - PubMed
-
- Enerbäck S (2010) Human Brown adipose tissue. Cell Metab 11:248–252. https://doi.org/10.1016/j.cmet.2010.03.008 - DOI - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials