Adipocyte lipolysis in normal weight subjects with obesity among first-degree relatives
- PMID: 8858214
- DOI: 10.1007/BF00403911
Adipocyte lipolysis in normal weight subjects with obesity among first-degree relatives
Abstract
In this study we investigated whether fat cell lipolysis could be involved in the aetiology of obesity by comparing non-obese subjects with (Hob) or without (Hnorm) a family trait for overweight. A family history of obesity was present when at least one of the first-degree relatives had body mass index of 27 kg/m2 or more. Twenty-seven healthy, drug-free non-obese adult subjects were investigated; 13 were Hob and the remaining 14 were Hnorm. Eleven Hob had at least one obese parent. Isolated fat cells from abdominal subcutaneous adipose tissue were incubated in vitro. Glycerol release (lipolysis index), mRNA levels and enzymatic activity of hormone-sensitive lipase and radioligand binding to beta 1- and beta 2-adrenoceptors were determined. The lipolytic effects of noradrenaline (major endogenous lipolytic agent), isoprenaline (a non-selective beta-adrenoceptor agonist), forskolin (a direct activator of adenylyl cyclase) and dibutyryl cyclic AMP (activating protein kinase and thereby hormone-sensitive lipase) were reduced by about 50% (p from 0.001 to 0.01). The maximum activity of hormone-sensitive lipase was reduced 50% in Hob (p < 0.05) and correlated with the lipolytic responsiveness of fat cells in the whole population (r = 0.71). However, there was no difference between the groups in steady-state mRNA levels for the enzyme. Beta 1-->, beta 2- and alpha 2-adrenoceptor sensitivity as well as beta 1- and beta 2-adrenoceptor numbers were normal in Hob. Fasting plasma insulin was 49.1 and 32.6 pmol/l, respectively in Hob and Hnorm (p = 0.01). There was, however, no significant correlation between lipolysis in vitro and plasma insulin. Thus, lipolytic catecholamine resistance in fat cells, at least partly due to impaired function of hormone-sensitive lipase, is an adipocyte abnormality associated with a family tendency to obesity.
Similar articles
-
Regulation of lipolysis in fat cells of obese women during long-term hypocaloric diet.Int J Obes Relat Metab Disord. 1996 Aug;20(8):745-52. Int J Obes Relat Metab Disord. 1996. PMID: 8856398
-
Catecholamine-induced adipocyte lipolysis in human hyperthyroidism.J Clin Endocrinol Metab. 1997 Jan;82(1):159-66. doi: 10.1210/jcem.82.1.3664. J Clin Endocrinol Metab. 1997. PMID: 8989252
-
Lipolytic catecholamine resistance linked to alpha 2-adrenoceptor sensitivity--a metabolic predictor of weight loss in obese subjects.Int J Obes Relat Metab Disord. 1997 Apr;21(4):314-20. doi: 10.1038/sj.ijo.0800407. Int J Obes Relat Metab Disord. 1997. PMID: 9130030
-
Genetic variance and lipolysis regulation: implications for obesity.Ann Med. 2001 Nov;33(8):542-6. doi: 10.3109/07853890108995964. Ann Med. 2001. PMID: 11730161 Review.
-
Regulation of beta 3-adrenoceptor expression in white fat cells.Fundam Clin Pharmacol. 1995;9(2):97-106. doi: 10.1111/j.1472-8206.1995.tb00268.x. Fundam Clin Pharmacol. 1995. PMID: 7628838 Review.
Cited by
-
P2Y13 receptor deficiency favors adipose tissue lipolysis and worsens insulin resistance and fatty liver disease.JCI Insight. 2024 Mar 12;9(8):e175623. doi: 10.1172/jci.insight.175623. JCI Insight. 2024. PMID: 38470490 Free PMC article.
-
Lipolysis defect in people with obesity who undergo metabolic surgery.J Intern Med. 2022 Oct;292(4):667-678. doi: 10.1111/joim.13527. Epub 2022 Jun 7. J Intern Med. 2022. PMID: 35670497 Free PMC article.
-
Obesity and hypovitaminosis D: causality or casualty?Int J Obes Suppl. 2019 Apr;9(1):20-31. doi: 10.1038/s41367-019-0010-8. Epub 2019 Apr 12. Int J Obes Suppl. 2019. PMID: 31391922 Free PMC article. Review.
-
Emerging Roles of Sympathetic Nerves and Inflammation in Perivascular Adipose Tissue.Cardiovasc Drugs Ther. 2019 Apr;33(2):245-259. doi: 10.1007/s10557-019-06862-4. Cardiovasc Drugs Ther. 2019. PMID: 30747398 Free PMC article. Review.
-
Potential effects of aerobic exercise on the expression of perilipin 3 in the adipose tissue of women with polycystic ovary syndrome: a pilot study.Eur J Endocrinol. 2015 Jan;172(1):47-58. doi: 10.1530/EJE-14-0492. Epub 2014 Oct 23. Eur J Endocrinol. 2015. PMID: 25342854 Free PMC article.