An improved method for the determination of green and black tea polyphenols in biomatrices by high-performance liquid chromatography with coulometric array detection
- PMID: 10706785
- DOI: 10.1006/abio.2000.4487
An improved method for the determination of green and black tea polyphenols in biomatrices by high-performance liquid chromatography with coulometric array detection
Abstract
Tea polyphenols are strong antioxidants and are believed to have beneficial health effects. However, the blood and tissue levels of these compounds are not well characterized because of a lack of suitable analytical methods for the biological resolution of these compounds. Previously, we developed methods for the analysis of three green tea catechins. Now we report an improved method for the measurement of the levels of the different catechins and theaflavins in biological fluids and tissues. The method includes digestion of the plasma, urine, or tissue samples with beta-d-glucuronidase and sulfatase, followed by extraction with ethyl acetate and subsequent separation by reversed-phase high-performance liquid chromatography (HPLC). The polyphenols are identified on the basis of their retention times, spectral analysis, and electrochemical behavior across an array of electrodes. In a single HPLC run, it is possible to determine the major catechins and theaflavins as well as some of the catechin metabolites. The detection limits for catechins and theaflavins are from 5 to 10 ng/ml of saliva, plasma, or urine.
Copyright 2000 Academic Press.
Similar articles
-
Antioxidative properties of black tea.Prev Med. 2005 Jun;40(6):910-8. doi: 10.1016/j.ypmed.2004.10.014. Prev Med. 2005. PMID: 15850895 Review.
-
Factors affecting the levels of tea polyphenols and caffeine in tea leaves.J Agric Food Chem. 2003 Mar 26;51(7):1864-73. doi: 10.1021/jf021066b. J Agric Food Chem. 2003. PMID: 12643643
-
[Chromatographic analysis of tea polyphenols].Se Pu. 2000 Jan;18(1):35-8. Se Pu. 2000. PMID: 12541451 Review. Chinese.
-
Simultaneous determination of all polyphenols in vegetables, fruits, and teas.J Agric Food Chem. 2003 Jan 29;51(3):571-81. doi: 10.1021/jf020926l. J Agric Food Chem. 2003. PMID: 12537425
-
Analysis of plasma and urinary tea polyphenols in human subjects.Cancer Epidemiol Biomarkers Prev. 1995 Jun;4(4):393-9. Cancer Epidemiol Biomarkers Prev. 1995. PMID: 7655336
Cited by
-
Catechin Bioavailability Following Consumption of a Green Tea Extract Confection Is Reduced in Obese Persons without Affecting Gut Microbial-Derived Valerolactones.Antioxidants (Basel). 2022 Dec 18;11(12):2490. doi: 10.3390/antiox11122490. Antioxidants (Basel). 2022. PMID: 36552698 Free PMC article.
-
The Effects of Green Tea on Diabetes and Gut Microbiome in db/db Mice: Studies with Tea Extracts vs. Tea Powder.Nutrients. 2021 Sep 10;13(9):3155. doi: 10.3390/nu13093155. Nutrients. 2021. PMID: 34579032 Free PMC article.
-
Characterizing relationship between chemicals and in vitro bioactivities of teas made by six typical processing methods using a single Camellia sinensis cultivar, Meizhan.Bioengineered. 2021 Dec;12(1):1251-1263. doi: 10.1080/21655979.2021.1903237. Bioengineered. 2021. PMID: 33904375 Free PMC article.
-
Immunomodulatory Effect of Green Tea Treatment in Combination with Low-dose Chemotherapy in Elderly Acute Myeloid Leukemia Patients with Myelodysplasia-related Changes.Integr Cancer Ther. 2021 Jan-Dec;20:15347354211002647. doi: 10.1177/15347354211002647. Integr Cancer Ther. 2021. PMID: 33754891 Free PMC article.
-
Monitoring the Diversity and Metabolic Shift of Gut Microbes during Green Tea Feeding in an In Vitro Human Colonic Model.Molecules. 2020 Nov 3;25(21):5101. doi: 10.3390/molecules25215101. Molecules. 2020. PMID: 33153091 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources